(2R,3R)-丁二醇脱氢酶的体外重组表达

Recombinant Expression of(2R,3R)-Butanediol Dehydrogenase in Vitro

目的明确2,3-丁二醇脱氢酶(2,3-BDH)如何具有不同的立体定向性。方法从枯草芽孢杆菌中克隆出(2R,3R)-丁二醇脱氢酶基因,将克隆的目的基因导入p MD18-T载体中,构建的重组质粒经测序后,将本实验序列和已发表序列进行比对分析,构建进化树。结果重组质粒可在重组菌中获得可溶性表达。结论利用重组菌株诱导表达获得了(2R,3R)-丁二醇脱氢酶。

ObjectiveTo establish how 2,3-BDH possesse differential stereo specificities.Methods The gene of（2R,3R）-BDH amplified from the genome of Bacillus subtilis,and was inserted into pMD18-T vector for sequencing. The constructed recombinant expression vector was sequenced, and the result of this experiment was compared with the known sequence to construct evolutionary tree.ResultsSoluble expression of the recombinant plasmid was obtained in the recombinant bacteria.Conclusion （2R,3R）-BDH was obtained by induced expression in recombinant bacteria.