放线菌代谢产物对蔬菜土传病原真菌的抑菌活性及毒力测定

Antimicrobial activity and virulence of actinomycetes metabolites against vegetable soil borne pathogenic fungi

采用平板对峙培养法、菌丝生长速率法等研究方法,测定了土壤放线菌代谢产物对蔬菜土传病原真菌的抑菌活性和毒力。结果表明:从宁夏10多个市县采集设施蔬菜等多种作物根部和不同植被的122份土壤样品中分离获得483个放线菌株,并进一步纯化、观测和确定了生长速度较快,长势良好的放线菌菌株212株,通过拮抗作用、抑菌活性和毒力测定,选择出了对蔬菜土传病害多种病原真菌具有显著抑制作用的生防放线菌优良菌株3种,为生物农药的开发提供良好菌源。

By means of plate confrontation culture and mycelium growth rate, the antimicrobial activity and virulence of metabolities of soil actinomycetes against vetetable soil borne pathogenic fungi were tested . The results showed that 483 actinomycete strains were isolated from 122 soil samples of protected vegetable, many other crop roots and different vegetatation from more than 10 cities and counties in Ningxia, and further purified, observed and determined 212 strains of actinomycetes with faster growth rate and we well growth. Through determination of the antagonism, antibacterial activity and virulence, 3 strains of biocontrol actinomyces which had significant inhibitory effect on many pathogenic fungi of vegetable soil borne diseases were screened out in order to provide good source of actinomycete strains for the development of biological pesticide.