蚕卵经盐酸浸渍后的卵壳蛋白变化分析

Analysis on eggshell protein changes after silkworm eggs soaked by HCl

对932品种在卵期发育和幼虫期饲养阶段实施温湿环境调控,让其产下滞育卵和非滞育卵。滞育卵经即时浸酸处理后,用扫描电镜观察卵壳的变化,可见卵壳表面比对照出现较多的裂痕。通过SDS-PAGE电泳技术,比较滞育卵、滞育卵经即时浸酸处理、非滞育卵的卵蛋白变化,发现滞育卵经浸酸处理后,20 ku以下的小分子量蛋白明显减少。利用shotgun技术对此类小分子量蛋白组分进行鉴定分析,发现在滞育卵和非滞育卵中都存在Er B.1、Er B.2、Er B.3、Hc-A和Hc-B卵壳蛋白,在经即时浸酸处理过的滞育卵中却未发现,由此推测蚕卵经浸酸处理后会引起这5种卵壳蛋白的降解。

Diapause and non-diapause eggs were laid by the temperature and humidity regulation in the egg and larvae feeding stage of 932 variety. Diapause eggs by HC1 treatment were observed more visible eggshell cracks than the control by scanning electron microscope. The total proteins of diapause eggs, diapause eggs by HC1 treatment and non- diapause eggs were compared using SDS-PAGE electrophoresis. Small molecular proteins below 20 ku decreased in the proteins of diapause eggs by HC1 treatment. The small molecular proteins were identified by shotgun analysis. The ErB.1, ErB.2, ErB.3, Hc-A and Hc-B eggshell proteins were detected in the diapause and non-diapause eggs, but were not detected in the diapause eggs by HC1 treatment. It is speculated that the diapause eggs by HC1 treatment may cause the degradation of the five eggshell proteins.