双波长HPLC法测定益心舒胶囊中4种成分的含量

Contents Determination of 4 Components in Yixinshu Capsule By Dual-wavelength HPLC

目的：建立测定益心舒胶囊中丹参素、原儿茶醛、阿魏酸、丹酚酸B含量的方法。方法：采用双波长高效液相色谱法。色谱柱为Eclipse XDB-C18,流动相为0.5%磷酸水溶液-甲醇-乙腈（梯度洗脱）,流速为1.0 ml/min,检测波长为280 nm（丹参素、原儿茶醛、丹酚酸B）、320 nm（阿魏酸）,柱温为30℃,进样量为10μl。结果：丹参素、原儿茶醛、阿魏酸、丹酚酸B质量浓度分别在9～144、0.5～8、0.65～10.4、221.25～3 540μg/ml范围内与各自峰面积呈良好的线性关系（r均为0.999 9）;精密度、稳定性、重复性试验的RSD均≤1.90%;平均加样回收率分别为100.8%、100.1%、100.1%、99.4%,RSD分别为1.65%、2.87%、3.01%、2.05%（n=9）。结论：该方法简单易行、重复性好,可用于益心舒胶囊的质量控制。

OBJECTIVE： To establish a method for the contents determination of tanshinol, protocatechuic aldehyde, ferulic ac- id and salvianolic acid B in Yixinshu capsule. METHODS： Dual-wavelength HPLC was performed on the column of Eclipse XDB-C18 with mobile phase of 0.5% phosphoric acid-methanol-acetonitrile （gradient elution） at the flow rate of 1.0 ml/min, the de- tection wavelength was 280 nm（tanshinol, protocatechuic aldehyde, salvianolic acid B） and 320 nm（ferulic acid）, column tempera- ture was 30 ℃ and volume was 10 μl. RESULTS：The linear range of tanshinol, protocatechuic aldehyde, ferulic acid and salvianolic acid B were respectively 9-144 μg/ml（r=0.999 9）,0.5-8 μg/ml（r=0.999 9）, 0.65-10.4μg/ml （r=0.999 9） and 221.25-3 540 μg/ml （r= 0.999 9）; RSDs of precision, stability and reproducibility tests were no more than 1.90%; the average recovery was respective- ly 100.8% （RSD=1.65% ,n=9）, 100.1% （RSD=2.87% ,n=9）, 100.1% （RSD=3.01% ,n=9） and 99.4% （RSD=2.05% ,n=9）. CONCLUSIONS： The method is simple and reproducible, and can be used for the quality control of Yixinshu capsule.