星点设计-效应面法辅助优选姜黄和甘草的提取方法

Optimization of Extraction Process of Three Curcuminoids in Curcumin Longae Rhizoma combined with Glycyrrhiza Uralensis Fisch in Extracting by Central Composite Design-Response Surface Methodology

目的:利用星点设计-效应面法优选姜黄和甘草共提的最佳提取方法。方法:比较超声提取、回流提取和渗漉法,确定提取方法后,根据星点设计安排表进行试验,HPLC法测定提取液中姜黄素(CUR)、去甲氧基姜黄素(D)和双去甲氧基姜黄素(BD)的含量,并算出总OD值,效应面法选出最佳条件。结果:渗漉法最好,渗漉最佳条件为90%乙醇浸泡30 h时,三者提取量最大。结论:验证试验证明,该优选方法可靠稳定适用。

Objective： Central Composite Design · Response Surface Methodology is used to optimizing of Extraction Process of three curcuminoids in Rhizoma Curcumin Longae combined with Glycyrrhiza uralensis Fisch in extracting. Methods： HPLC is used to determine the contents of Cureumin （ CUR）, Demethoxycurcumin （D） ＆Bisdemethoxycurcumin （ BD）, calculating the OD to chose the best method. Results： Percolation is the best extraction technology with its conditions： 90% ethanol and 30h soak time. Conclusion： He selected extrTaction method is steasy, reliable and suitable for use.