海洋石油烃降解菌的降解性能与固定化研究

Characterization and Immobilization of Marine Petroleum Hydrocarbon Degrading Bacteria

对2株海洋石油烃降解菌(HD-1和HD-2)进行了研究;采用气相色谱法分析了这2株菌对正构烷烃组分的降解情况。结果表明HD-1菌株对正构烷烃的降解性能优于HD-2菌株。碳链的长度对降解率有显著影响。C11能分别被HD-1和HD-2降解90.9%和73.8%,但C25却只能被降解73.3%和27.7%。采用麦饭石、孔质砂和稻壳碳固定混合菌液后,对培养液中柴油的去除率都明显高于未固定化的菌液的去除率,其中麦饭石固定化菌的去除率最高,达到98%。

Two petroleum hydrocarbon degrading bacteria strains（ named HD-1 and HD-2） were studied. Gas chromatography was performed on n-alkanes degradation. The most efficient n-alkanes degrader was HD-1. The chain length influenced n-alkanes biodegradation to a significant extent. C11 was almost completely（ 90. 9%,HD-1） and highly（ 73. 8%,HD-2） degraded. The initial amount of C25,27. 7%（ HD-2） or 73. 3%（ HD-1） were degraded. About 98% oil was degraded by immobilized strains which were adsorbed by medical stones.