肝细胞生成素及肝部分切除快速诱导肝再生相关基因Tec的表达

Rapid induction of mRNAs for liver regeneration genes by hepatopoitin and partial hepatectomy

目的 肝部分切除术后肝细胞生成素 (HPO)迅速增加 ,该文观察人重组肝细胞生成素(rhHPO)和肝部分切除 (PH)迅速诱导瞬时早期反应基因表达情况。方法 将Wister大鼠分为PH组和对照组 ,每组 3只。利用表达性差异显示分析技术和序列分析 ,检测 2 /3PH后 1h及原代培养大鼠肝细胞体系的选择性基因表达。结果 EST库中的大部分为瞬时早期反应基因 ,发现一种新的可能与肝再生调控相关的Tec基因 ,Northern杂交证实 2 /3PH可迅速诱导Tec基因的表达 ,其表达高峰为术后 1～ 2h。HPO在原代培养大鼠肝细胞体系中可迅速诱导瞬时早期反应基因 (c fos、LRF 1和Tec等 )表达。结论 HPO和PH可迅速诱导瞬时早期反应基因表达 ,首次报道Tec基因是一种与肝再生调控密切相关的早期反应基因。

Objective After partial hepatectomy, hepatopoietin(HPO) increase rapidly. The present study was designed to investigate how rhHPO and partial hepatectomy induce the expression of immediate early gene. Methods The Wister rats were divided into hepatectomy group and control group. The different gene expression within 1 h after 2/3 partial hepatectomy and in primary cultured hepatocytes system were examined by using representational difference analysis. Results In the expressed sequence tag(EST) library, most of these genes were identified as immediate early genes, and one new gene Tec that might be associated with liver regeneration in the EST library was found. Moreover, the Tec gene was rapidly induced by 2/3 partial hepatectomy. The expression peak achieved within 1 2 h after operation. HPO can rapidly induce the expression of these genes(c fos, LRF 1 and Tec, etc.) in primarily cultured rat hepatocytes. Conclusion rhHPO and partial hepatectomy can rapidly induce the expression of immediate early gene. This is the first report that Tec gene is an immediate early gene related to liver regeneration.