肿瘤患者外周血CD4^+和CD8^+ T淋巴细胞TCR Vβ基因克隆化分析

Analysis on T Cell Receptor Vβ Gene Cloning in Peripheral CD4^+ and CD8^+ T Lymphocytes in Cancer Patients

目的探讨肿瘤患者T细胞抗原受体(TCR)Vβ基因克隆化改变特征.方法应用多引物巢式PCR技术检测肿瘤患者外周血CD4+T细胞和CD8+T细胞TCR Vβ基因22个亚家族的克隆化改变,与正常对照组比较,分析肿瘤患者TCR单克隆改变的特点.结果 CD8+T细胞TCRVβ基因亚家族单克隆改变的数量多于CD4+T细胞;肿瘤患者的CD4+T细胞中,只有Vβ2,Vβ7和Vβ8三个亚家族单克隆改变高于正常对照组(P<0.01或P<0.05),其他Vβ亚家族单克隆改变与正常对照组无明显差异.肿瘤患者的CD8+T细胞中,有14个Vβ亚家族单克隆改变均高于正常对照组(P<0.01或P<0.05);4组肿瘤患者中,CD4+T细胞和CD8+T细胞的TCR Vβ7亚家族的单克隆改变均明显高于正常对照组(P<0.01或P<0.05).结论通过检测TCR Vβ基因克隆化改变,可以初步了解T细胞对肿瘤的免疫应答状况.

Objective To investigate the character of T cell antigen receptor（TCR） Vβ gene cloning changes in the patients. Method Muhi-primers nested PCR technique was applied to detect the cloning changes of 22 subfamily of CD4＋ T cells and CD8＋ T cell TCR Vβ gene in tumor patients. The results were compared with the normal control group, and the TCR monoclonal change features in cancer patients were analyzed. Results The number of CD8＋ T cells TCR Vβ gene subfamily monoclonal change was more than that of CD4＋ T cells. In the tumor patients CD4＋ T cells, only three subfamilies （ Vβ2, Vβ7 and Vβ8 ） monoclonal changes were higher than that in the control group（ P〈0.01 or P〈0.05 ）. As for other Vβ subfamily monoclonal changes, no significant difference was observed compared with the control group. For CD8＋ T cells of the tumor patients, there were 14 Vβ subfamilies monoelonal change were higher than that of the normal control group （P〈0.01 or P〈0.05 ）. Among of four groups of tumor patients, Vβ7 subfamilies monoclonal changes in both CD4＋ T cells and CD8＋T cell TCR were significantly higher than that of the control group （ P 〈 0.01 or P 〈 0.05 ）. Conclusion By detecting TCR Vβ gene monoclonal change, the situation of T cell immune response to the tumor could be preliminary understood.