摘要
目的 肿瘤复发转移仍是威胁患者生存的重要原因 ,及早检出外周血中的循环肿瘤细胞并进行有效的综合治疗有着重要的临床意义。检测结、直肠癌患者外周血中癌胚抗原 (CEA) mRNA的表达及血清CEA蛋白的含量 ,并分析其临床价值。方法 35例有明确临床肿瘤病灶存在的结直肠癌患者、9例根治术后患者和 37例健康志愿者 ,分别以逆转录PCR(RT PCR)检测其外周血中CEA mRNA的表达 ,时间分辨荧光方法测定其血清CEA蛋白的含量。结果 35例结直肠癌患者CEA mRNA阳性率为 4 5 .7% ,而健康对照组阳性率为 2 .7% ,两者比较差异有显著性 (P <0 .0 5 ) ;9例术后患者CEA mRNA仅 2例阳性 ;CEA mRNA阳性表达率与肿瘤分期相关 ,与肿瘤细胞分化程度并不相关。CEA mRNA阳性组血清CEA蛋白阳性率 (72 .2 % )显著高于CEA mRNA阴性组 (42 .3% )。结论 外周血细胞中CEA mRNA的表达与肿瘤分期相关 ,有可能作为结直肠癌微转移的一个监测指标。长期的随访有助于评价其临床价值。
Objective The recurrence and metastasis of malignant tumor could affect the survival of patients. Early detection of cancer cells in peripheral blood and effective therapy promptly are of clinical importance. The present study was to investigate the carcinoembryonic antigen (CEA) mRNA expression and serum CEA level in blood from patients with colorectal cancer and to evaluate its clinical significance. Methods Peripheral blood samples were collected from 35 eligible patients with colorectal cancer and 9 post operative patients. CEA mRNA was detected by reverse transcriptase polymerase chain reaction (RT PCR) and serum CEA was detected by time resolved fluorometric methods. Results The positive rate of CEA mRNA in blood samples of 35 patients with colorectal cancer was 45.7%, and 2.7% in healthy volunteers. Two of the nine post operative patients (22.2%) showed CEA mRNA positive. The positive rate increases with the pathological staging and showed no significant relation with the malignant extent of cell differentiation. Serum CEA mRNA positive rates in patients with CEA positivity (72.2%) were significantly higher than those in patients with negative CEA mRNA expression (42.3%).Conclusion The expression of CEA mRNA in peripheral blood cells correlates with the pathological staging of colorectal cancer and it could be of potential use to monitor the micrometastases of tumor. Long term follow up is needed to evaluate its clinical significance.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2002年第8期485-488,共4页
Chinese Journal of Digestion
关键词
直肠癌
循环肿瘤细胞
微转移
癌胚抗原
逆转录聚合酶链反应
结肠癌
Colorectal cancer
Circulating tumor cells
Micrometastases
Carcinoembryonic antigen
Transcriptase polymerase chain reaction