双特异性磷酸酶5基因在良性前列腺增生症与前列腺癌中的表达及临床意义

Expression and clinical significances of DUSP5 in prostate cancer tissues and benign prostate hyperplasia tissues

目的前期研究发现双特异性磷酸酶5(DUSP5)基因在良性前列腺组织和前列腺癌组织中存在差异性表达,但DUSP5基因的表达与前列腺癌疾病的临床病理相关性尚无统计研究。本次研究检测DUSP5在良性前列腺增生和前列腺癌中的表达,分析其表达水平与临床病理特征的关系。方法通过RT-qPCR检测DUSP5在前列腺癌、前列腺增生组织中DUSP5基因的表达情况;免疫组化检测前列腺癌、前列腺增生标本中DUSP5蛋白的表达。分析DUSP5的表达水平与临床病理特征的关系。结果前列腺癌患者组织中DUSP5基因表达显著下调,DUSP5蛋白表达下调与肿瘤临床分期(≤T2期vs.>T2期:6.43±0.96 vs.5.10±0.70,P<0.01)、Gleason评分(GS<7 vs.GS≥7:6.37±0.78 vs.5.04±0.57,P<0.01)显著相关。结论 DUSP5蛋白在前列腺癌组织中表达下调,检测DUSP5表达水平可协助判断前列腺癌分化程度并评估预后。

Objective To study the expression and significances of DUSP5 in benign prostate hyperplasia （BPH） tissues and prostate cancer tissues, and to illustrate the correlations with clinical significances. Methods RT-qPCR was used to detect DUSP5 protein in prostate cancer tissue and BPH tissue, immunohistochemical method was used to detect DUSP5 in prostate cancer tissue and BPH tissue, and evaluated the correlations with clinical Significances of prostate cancer. Results The expression of DUSP5 mRNA was significantly down-regulated in prostate cancer tissues. The expression level of DUSP5 was statistically significant correlated with clinical stage, Gleason score. Conclusion Our study showed the expression of DUSP5 had down-expression changed trend, suggesting the low level of DUSP5 may help to diagnosis pathologic grade and predict the prognosis of prostate cancer.