母婴分离对小鼠行为学及胶质纤维酸性蛋白表达的影响

Effect of maternal separation on behavior and glial fibrillary acidic protein expression in C57BL/6N Mice

目的探讨母婴分离对小鼠行为学及胶质纤维酸性蛋白（GFAP）表达的影响。方法 9只C57BL/6N孕小鼠随机分为3组：对照组、MS15组和MS180组,每组F0代母鼠3只;所产F1代小鼠,分别于出生第2~21天进行母婴分离,母婴分离时先将F0代母鼠隔离至单独鼠笼,将F1代子鼠移至孵育箱中。MS15组母婴分离时间9：00—9：15 am,MS180组分离时间9：00—12：00 am,对照组不分离。采用旷场实验评价母婴分离对小鼠自发活动的影响,强迫游泳和强迫悬尾实验评价母婴分离对小鼠不动时间的影响,Western blot免疫印迹法检测GFAP蛋白表达,Real-time PCR检测GFAP mRNA表达。结果旷场实验中各组小鼠活动总路径差异无统计学意义（P〉0.05）。与对照组及MS15组比较,MS180组在强迫游泳实验、强迫悬尾实验中,小鼠不动时间显著增加（P〈0.01）;GFAP蛋白及mRNA表达显著升高（P〈0.01）。结论母婴分离对小鼠行为有显著影响,小鼠出现抑郁样行为,GFAP蛋白和mRNA水平都升高。

Objective To investigate the effects of maternal separation on C57BL/6N mice behavior and glial fi- brillary acidic protein （GFAP） expression. Methods Nine pregnant C57BL/6N mice were randomly assigned to one of the following three groups, control, MS15 and MS180. There were 3 pregnant mice in each group. The F1 offspring were separated from their mothers at Day 2 to Day 21 after birth. Maternal separation was implemented by isolating F0 in a soli- tary cage and allocating F1 to an incubator. Effects of maternal separation on spontaneous activity were evaluated by field test while effects on immobility time by forced swimming test and tail suspension test. GFAP expression was detected by Western blot. Expression of GFAP mRNA was detected by real - time PCR. Results No statistically significant difference was found in total animal activity path in field test （P 〉 0. 05 ）. Compared with the control group and MS15 group, immo- bility time in the MS180 group significantly increased （P 〈 0. 01 ） in the forced swimming test and tail suspension test; ex- pression of GFAP protein and mRNA increased significantly in the MS180 group （P 〈 0.01 ） as well. Conclusion Ma- ternal separation has a significant influence on the behavior of mice. Depressive - like behavior and increased GFAP ex- pression were found in mice undergoing maternal separation.