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姜黄素和穿心莲内酯对鼻咽癌细胞CNE-2的作用及机制 被引量：2

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摘要 目的研究姜黄素和穿心莲内酯对人鼻咽癌细胞CNE-2体外抗癌的联合作用,并探讨其可能抗癌分子机制。方法 MTS法检测穿心莲内酯(Andro)单独作用以及与姜黄素(Cur)的联合作用(DMSO、Andro 5.0μmol/L、Andro 10.0μmol/L、Cur 10.0μmol/L、Andro 5.0μmol/L+Cur 10.0μmol/L、Andro 10.0μmol/L+Cur 10.0μmol/L)对不同肿瘤细胞增殖的影响;流式细胞术分析姜黄素和穿心莲内酯联合作用对CNE-2和He La细胞周期影响;Wes-tern blot分析姜黄素和穿心莲内酯联合作用对CNE-2细胞的NF-κB家族相关蛋白P50和P65蛋白的表达。结果穿心莲内酯单独作用时在10μmol/L以下浓度对各肿瘤细胞无显著抑制作用,而10μmol/L姜黄素联合穿心莲内酯处理CNE-2和He La细胞48 h能使细胞存活率显著降低以及使细胞发生明显的G2/M期阻滞;Western blot结果显示两者联合作用能够协同抑制NF-κB家族相关蛋白P50和P65以及NF-κB调控蛋白c-Myc的表达。结论姜黄素和穿心莲内酯协同作用于NF-κB信号通路发挥抗肿瘤作用。
作者 吴清清 陈永恒 廖芬芳 王伟章
机构地区 广东药学院基础学院
出处 《广东医学》 CAS 北大核心 2015年第15期2327-2330,共4页 Guangdong Medical Journal
基金 国家自然科学基金资助项目(编号:81100369) 广东省科技计划项目(编号:2013B021800084)
关键词 姜黄素 穿心莲内酯 CNE-2 NF-ΚB
分类号 R739.63 [医药卫生—肿瘤]
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广东医学
2015年 第15期

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