昆母汤对类风湿关节炎滑膜细胞增殖及凋亡的影响

Effects of Kunmu decoction on proliferation and apoptosis of fibroblast-like synoviocytes in rheumatoid arthritis

目的:观察昆母汤醇提液对人类风湿关节炎(RA)成纤维样滑膜细胞(FLS)增殖及凋亡的影响,探讨昆母汤治疗RA的部分作用机制。方法:滑膜组织取自行关节置换术或关节镜检查术的8例活动性RA患者,分离出FLS进行原代培养,采用流式细胞术检测RA滑膜细胞表面抗原,应用MTT比色法检测醇提液对RA-FLS细胞的生长抑制作用,流式细胞术测定细胞凋亡率。结果:原代培养的RA滑膜细胞中CD90+阳性细胞为(94.78±0.98)%。醇提液对RA-FLS具有生长抑制作用,且具有一定的时间-浓度依赖性。醇提液和甲氨蝶呤均可显著促进FLS的凋亡,且醇提液促FLS凋亡能力在一定程度上呈剂量依赖性。结论:昆母汤可抑制RA-FLS增殖、促进其凋亡,具有治疗RA的潜在价值。

Objective To investigate the effects of alcohol extract of Kunmu decoction on proliferation and apoptosis of rheumatoid arthritis fibroblast-like synoviocytes （RA-FLS）. Methods Synovial tissues were obtained from patients with active RA received joint replacement or arthroscopy. The surface antigen and the amount of apoptotic cells were determined by flow cytometry. The inhibitive effect was detected by MTT assay. Results The CD90＋ surface antigen of synoviocytes was （94.78 ± 0.98）%. The inhibitive effect on the proliferation in all treatment groups were in a time-and dose-dependent manner. The apoptosis rate was increased in a dose- dependent manner among all dosage alcohol extract groups. Conclusion Kunmu decoction might inhibit proliferation and induce apoptosis of RA-FLS.