血管内皮生长因子受体2在痔黏膜中的分布特征及临床意义

Clinical significance and distribution characteristics of expression of vascular endothelial growth factor receptor 2 in hemorrhoid mucosa

目的:观察血管密度与血管内皮生长因子受体2(VEGFR2)在痔黏膜及黏膜下组织的表达,探讨VEGFR2在痔病发生机制中的作用。方法:痔患者组随机选取60例,正常对照组20例。测定痔患者和对照组的肛管静息压(RASP)、肛管收缩压(MASP)、直肠感知阈值(RSTV)、直肠最大容量阈值(RMTV),免疫组化检测组织中VEGFR2蛋白表达水平和微血管密度(MVD),RT-PCR方法检测组织中VEGFR2 m RNA的表达丰度。结果:痔组织与正常肛垫相比存在着明显的新生血管形成,痔组织与对照组中的MVD均值分别为5.59±0.98、3.92±0.81(P<0.05);VEGFR2染色评分分别为5.95±1.01、2.32±0.72(P<0.05),痔组织中VEGFR2 m RNA表达丰度明显强于对照组(P<0.05)。结论:VEGFR2在痔垫的微血管生成发展中起着重要的作用,可能与痔病的发生及临床症状密切相关。

Objective To investigate the micro-vessel density and vascular endothelial growth factor receptor 2 （VEGFR2） expression in mucosa and submucosa tissue of the patients with hemorrhoids. Methods Sixty hemorrhoids patients were randomly recruited and involved into experiment group and 20 cases of normal patients involved in control group. RASP, MASP, RSTV, RMTV, Expression of VEGFR2 and microvessel density in two groups were detected by immunohistochemistry. The expression abundance of VEGFR2 mRNA was detected by RT-PCR. Results Excessive angiogenesis was found in hemorrhoids tissue （5.59 ± 0.98） in experiment group as compared with that in control group （3.92 ± 0.81）, the difference was statistically significant （P 〈 0.05）. Staining score of hemorrhoid tissue and the control tissue were 5.95±1.01, 2.32 ± 0.72, respectively （P 〈 0.05）. Abundance of VEGFR2 mRNA expression in hemorrhoid tissue was significantly higher than that in control tissue （P 〈 0.05）. Conclusion VEGFR2 plays an important role in angiogenesis during the development of hemorrhoids, which may be closely related to the occurrence of hemorrhoid disease and clinical symptoms.