基于EST-SSR标记的莲属种质资源遗传多样性分析

Genetic Diversity Analysis of Nelumbo Accessions Based on EST-SSR Markers

利用EST—SSR分子标记对30个亚洲莲、6个美洲莲及14个亚美杂交莲品种（野生居群）进行遗传多样性分析，结果表明：从123对EST—SSR引物中筛选出52对（42．3％）扩增稳定、具有多态性的引物；利用这52对引物对50个不同类型代表的莲属品种进行扩增，共获得177条多态性条带，引物的等位基因数和多态性信息量（PIC）的范围分别为2～8个和0．63（NNFB-1059）-0．91（NNFB-750），平均为3．4个和0．79。利用NTSYS—pc2．11软件对扩增结果进行Jaccard相似性系数分析，50个荷花品种材料遗传相似系数为0．24～0．86；通过UPGMA法进行聚类分析，在遗传相似系数0．37处供试荷花材料可分为4大类群：亚洲莲品种均聚类在I、II类群中，III类群绝大部分为亚美杂交莲品种，IV类群为美洲莲，亚美杂交莲品种与亚洲莲品种的亲缘关系相对较近，与传统分类及前人研究结果相一致。

EST-SSR markers were applied to estimate the genetic diversity for 30 accessions of Nelumbo nucifera, 6 accessions of Nelumbo lutea and 14 hybrids between these two species. The 52 of 123 EST-SSR markers （42.3%） were screened and then applied to amplify the 50 Nelumbo accessions. A total of 177 alleles were identified, and the number of alleles per locus and Polymorphic Information Content （ PIC ） value varied from 2 to 8 with an average of 3.4 alleles and from 0. 63 （NNFB-1059） to 0. 91 （NNFB-750） with an average value of 0. 79, respectively. Jaccard similarity coefficients of the amplification results were analyzed by NTSYS-pc2.11 software and the genetic similarity coefficient was from 0. 24 to O. 86. The clustering dendrogram constructed by UPGMA method indicated that 50 ac- cessions of Nelumbo could be divided into four major groups at the similarity coefficient of 0. 37. Group I and group II included N. nucifera; group III included the majority of Asian-American lotus hybrids; and group IV included N. lutea. In addition, the Asian-American hybrids were closer to N. nucifera based on genetic relationship, which is consistent with the traditional classification result and the previous reports.