木兰科主要属种核DNA含量(2C-值)的检测

The Determination of Nuclear DNA Content(2C-value) on Some Representative Genus and Species of Magnoliaceae

植物核DNA含量(2C-值)作为描述生物多样性的一个主要特征参量,对植物资源的研究具有重要意义。本文选取木兰科(Magnoliaceae)中10个代表性属,即木兰属(Magnolia)、含笑属(Michelia)、木莲属(Manglietia)、鹅掌楸属(Liriodendron)、盖裂木属(Talauma)、合果木属(Paramichelia)、观光木属(Tsoongiodendron)、华盖木属(Manglietiastrum)、单性木兰属(Kmeria)和拟单性木兰属(Parakmeria)下的23个种;以玉米(Zea mays)核DNA含量(5.45 pg/2C)为标准,通过流式细胞术测定各样品的核DNA含量(2C-值)。结果表明,23个种的核DNA含量从3.25 pg(3 178.50 Mbp)到13.61 pg(13 310.58 Mbp)不等;且CV值均<5%。本研究结果可为木兰科中主要属下单元基因组大小的评估和其他植物核DNA含量的测定提供参考,同时也为木兰科植物资源的保护和利用研究奠定基础。

The plant nuclear DNA content （2C-value） is a principal characteristic parmeter to describe biodiversity of species, which has important significance on the study of plant resources. In this study, we choosed 23 species from 10 representative genera （Magnolia, Michelia, Manglietia, Liriodendron, Talauma, Paramichelia, Tsoon- giodendron, Manglietiastrum, Kmeria, Parakmeria） of Magnoliaceae in China. All samples were determined using a flow cytometry technique with a standard of Zea mays （ 5.45 pg/2C）. The amount of nuclear DNA among these species ranged from 3.25 pg （3 178.50 Mbp） to 13.61 pg （ 13 310. 58 Mbp） for P. grandiflora, M. officinalis subsp. biloba respectively, and the coefficient of variation （CV） were less than 5%. The results of the study will not only provide references for determination of the nuclear DNA content of Magnoliaceae and other plants, but also lay the foundation for the utilization and conservation of Magnoliaceae plant resources.