脂联素对脓毒症大鼠肝组织NLRP3炎性小体及炎症因子表达的影响

The effect of adiponectin on the expression of NLRP3 inflammasome and inflammatory factors in liver tissue of septic rats

目的 探讨脂联素对脓毒症大鼠肝组织NLRP3炎性小体及炎症因子表达的影响。方法清洁级7～8周龄雄性Wistar大鼠30只,随机均分为三组（n=10）：假手术组（S组）、模型组（M组）和脂联素组（APN组）。采用盲肠结扎穿孔（CLP）法建立脓毒症模型。S组剖腹后仅分离盲肠远端,不结扎穿孔。APN组于CLP前12h腹腔注射基因重组脂联素6mg/kg。CLP后24h处死,留取血样本后取肝组织标本。活性氧（ROS）的含量采用流式细胞技术测定、酶联免疫吸附法测定IL-1β水平、Western Blot检测NLRP3及caspase-1含量。结果 与S组比较,M组和APN组大鼠血清IL-1β水平明显升高、肝组织ROS、NLRP3及caspase-1含量明显增加（P〈0.05）;与M组比较,APN组大鼠血清IL-1β水平明显降低,肝组织ROS、NLRP3及caspase-1含量明显减少（P〈0.05）。结论脂联素可改善脓毒症大鼠炎性反应,其机制可能与降低ROS含量、抑制NLRP3炎性小体活性有关。

Objective To investigate the effect of adiponectin on the expression of NLRP3 in- flammasome and inflammatory factors in liver tissue of septic rats. Methods Thirty healthy male Wistar rats, aged 7-8 weeks were randomly divided into 3 groups （n = 10） ： group sham operation （group S）, group model （group M） and group adiponectin （group APN）. Septic model was per- formed by cecum ligation and puncture （CLP）. Group S only took the operation of isolated distal cae- cum, without ligation perforation. In group APN, gene recombinant adiponectin 6 mg/kg was injec- ted intraperitoneally at 12 h before CLP. Rats were been executed 24 h after CLP, liver tissue were harvested after obtained the blood samples. Flow cytometry was used for determining the level of Re- active oxygen species （ROS）, ELISA was performed for the level of IL-1β, Western Blot was used for the levels of NLRP3 and caspase-1. Results Compared with group S, the levels of serum L-1β and ROS, NLRP3, caspase-1 content in liver tissues were significantly increased in group M and group APN （P〈0. 05）; Compared with group M, the levels of serum L-1β and ROS, NLRP3, caspase-1 content in liver tissues were significantly decreased in group APN （P〈0. 05）. Conclusion Adiponec- tin might regulate immune response in septic rats by reducing the level of ROS and inhibiting the NL- RP3 inflammasome activity.