IL-6和IL-13与EV71型肠道病毒感染手足口病患者发病的相关性

Enterovirus 71 infection induced HFMD related to IL-6 and IL-13 level

目的观察肠道病毒71型(EV71)感染手足口病(HFMD)患者血清白细胞介素(IL)-6和IL-13水平变化情况及其临床意义,并对血清IL-6和IL-13细胞来源进行初步的探讨。方法采用酶联免疫吸附试验(ELISA)法测定HFMD患者和健康对照血清中IL-6和IL-13水平,比较不同严重程度的HFMD患者血清中IL-6和IL-13水平,并同时检测HFMD患者治疗后血清IL-6和IL-13水平变化情况。流式细胞术(FCM)分析外周血单个核细胞(PBMC)中单核细胞分泌IL-6和T细胞分泌IL-13的情况。EV71病毒感染健康人单核细胞和T细胞后,FCM检测单核细胞分泌IL-6和T细胞分泌IL-13变化情况。结果 EV71感染HFMD患者血清IL-6和IL-13水平分别为(79.63±29.45)pg/mL和(36.45±15.13)pg/mL,健康对照组血清IL-6和IL-13水平分别为(27.26±7.82)pg/mL和(13.46±3.14)pg/mL,HFMD患者血清IL-6和IL-13水平显著高于健康对照者(P<0.01),同时,随着HFMD患者疾病严重程度增加,血清IL-6和IL-13水平亦逐渐升高(P<0.01)。治疗后HFMD患者血清中IL-6和IL-13水平分别为(48.23±23.14)pg/mL和(23.25±9.63)pg/mL,与治疗前相比显著降低(P<0.01)。HFMD患者外周血单核细胞分泌IL-6水平和T细胞分泌IL-13的水平与健康对照者相比显著升高;EV71病毒处理健康人单核细胞和T细胞后单核细胞和T细胞分泌IL-6和IL-13能力显著升高。结论 EV71病毒可能时通过促进单核细胞分泌IL-6和T细胞分泌IL-13,致使血清IL-6和IL-13水平升高,从而参与HFMD的发生和发展。

Objective To study the clinical significance of interleukin（IL）-6and IL-13 levels in serum of hand,foot,and mouth disease（HFMD）caused by enterovirus 71,and carries on the preliminary discussion on serum IL-6and IL-13 cell origin.Methods Serum levels of IL-6and IL-13 in HFMD and healthy control were detected by enzyme linked immunosorbent assay（ELISA）,serum IL-6and IL-13 levels among different severity of HFMD were compared.Serum IL-6and IL-13 levels in HFMD after treatment were also detected.The secretion of IL-6by monocyte and the secretion of IL-13 by T cells in peripheral blood mononuclear cells（PBMC）were detected by Flow Cytometry（FCM）.Monocyte and T cells from health individual were treated with EV71,and the secretion of IL-6by monocyte and the secretion of IL-13 by T cells were detected by FCM too.Results Serum levels of IL-6and IL-13were（79.63±29.45）pg/mL and（36.45±15.13）pg/mL respectively in HFMD,and serum levels of IL-6and IL-13were（27.26±7.82）pg/mL and（13.46±3.14）pg/mL respectively in health control,serum IL-6and IL-13 levels in HFMD were significantly higher than that of healthy subjects（P〈0.01）,and the mean serum IL-6and IL-13 levels of HFMD were increased with the severity of the disease（P〈0.01）.After treatment,serum levels of IL-6and IL-13were（48.23±23.14）pg/mL and（23.25±9.63）pg/mL respectively,when compared with before treatment,serum levels of IL-6and IL-13 were significantly decreased（P〈0.01）.The secretion ability of IL-6by monocyte and the secretion of IL-13 by T cells in HFMD were significantly higher than that of healthy subjects.After infection by EV71,the secretion ability of IL-6by monocyte and the secretion of IL-13 by T cells were significantly increased.Conclusion Infection of EV71 probably by increase the secretion ability of IL-6by monocyte and the secretion of IL-13 by T cells,resulted in elevated of serum IL-6and IL-13 levels,which involved in the occurrence and development of HFMD.