铁皮石斛种质亲缘关系的ISSR分析

Analysis of Genetic Diversity of Germplasm Resources of Dendrobium officinale by ISSR

利用ISSR技术对36份的铁皮石斛种质资源进行亲缘关系分析。从备选72条引物中筛选出12条引物,共扩增出54条谱带,其中51条为多态性带,多态性比例为94.4%。聚类结果显示,36份铁皮石斛种质资源遗传相似系数在0.4062~0.9375。以遗传相似系数为0.70,可将铁皮石斛种质明显划分为6个类群。结果表明,铁皮石斛植物遗传多样性丰富,ISSR技术可应用于铁皮石斛的亲缘关系研究。

Genetic relationships of 36 Dendrobium officinale were studied by ISSR technique. 12 primers which could produce clear and the polymorphic bands were screened out from 72 ISSR random primers,54 locis were detected,of which 51 were polymorphic,and the polymorphic percentage was 94. 4 %. The genetic similarity of 36 Dendrobium officinale ranged from 0. 4062- 0. 9375,and 36 Dendrobium officinale were grouped into 6 sub-cluster at similarity coefficient of 0. 70. The result showed that the plants of Dendrobium officinale had rich genetic diversities,and ISSR technique could be used to study their genetic relationships.