奥美拉唑对小鼠胸腺、脾脏免疫功能的影响

Effects of omeprazole on thymus and spleen immunity in mice

目的研究长期使用奥美拉唑(omeprazole,OME)是否会抑制小鼠体内溶酶体及其对小鼠免疫器官的影响。方法实验分为单纯对照组、OME低剂量组(6 mg·kg-1)、OME高剂量组(30 mg·kg-1),每组10只小鼠。饲养24周后处死小鼠,ELISA法检测小鼠血清及脾脏中酸性磷酸酶(acid phosphatase,ACP)、N-乙酰-β-D-氨基葡萄糖苷酶(N-acety L-β-D-glucosaminidase,NAG)含量,采集小鼠胸腺、脾脏并称重计算小鼠脾脏、胸腺重量指数。结果 OME组与对照组相比血清中溶酶体酶活性下降,两者差异具有统计学意义(P<0.05),同时小鼠脾脏及胸腺重量指数明显下降。结论 OME可以抑制小鼠体内溶酶体及其水解酶的活性,导致系统性免疫功能受损,从而降低小鼠免疫功能。

Objective To investigate if long-term use omeprazole（ OME） will inhibit lysosomal in vivo in mice and its effect on the im-mune organs of mice.Methods Mice were divided into 3 group：control group,low dose OME group,high dose OME group,10 mice in each group.After 24 wk mice were sacrificed to collect samples and calculate the spleen index,ELISA was employed to assay the serum concentration of acid phosphatase（ACP）and N-acetyl -β-D-glucosaminidase（NAG）.Results OME group compare with the control group,spleen weight index,thymus index decreased significantly,serum lysosomal enzyme levels also decreased significantly,the differences was satatically significant（P〈0.05）.Conclusion OME can inhibit the lysosomal hydrolase activity in mice,resulting in systemic immune function is impaired,thereby reducing immune function in mice.