Roche cobas 6000生化免疫一体机在急诊留观感染患者检测中的应用

Application of the combined determination of procalcitonin and C-reactive protein by Roche cobas 6000 biochemical immune detector in patients infected in emergency observation

目的:探讨血清降钙素原(PCT)、C反应蛋白(CRP)及白细胞(WBC)在急诊留观感染患者中的鉴别诊断价值和联合诊断的意义。方法:选取急诊科留观收治的65例患者,将其分为感染组(44例)和非感染组(21例),同期选取40例健康体检者为对照组。采用Roche cobas 6000生化免疫一体机检测三组的PCT、CRP及WBC水平。结果:1PCT水平检测结果,感染组明显高于非感染组和健康对照组,差异有统计学意义(F=6.73,P<0.01);感染组、非感染组之间CRP、WBC水平无差异,但均明显高于对照组,差异有统计学意义(F=7.77,F=5.49;P<0.05);2PCT、CRP和WBC对感染组诊断的灵敏度分别为93.18%,75.01%和43.18%,PCT与CRP联合诊断感染组的灵敏度可达97.73%。结论:PCT指标在判断急诊留观患者感染时优于CRP和WBC指标,PCT与CRP联合检测时可以提高诊断急诊留观患者感染的灵敏度。

Objective：To evaluate the clinical significance of the combined determination of procalcitonin（PCT）,high-sensitivity C-reactive protein（CRP）, white blood cell（WBC） in emergency observation of infection in the differential diagnosis of patients and the diagnosis of joint.Methods： Sixty-five cases （Infection group and non-infection group） with infected in emergency observation selected from October 2014 to March 2015,Then select the healthy persons at the same period as the control group（40 cases）,to detect PCT, CRP and WBC levels. Results： （1）PCT infection levels were significantly higher than uninfected and healthy group, and the difference was statistically significant（F=6.73,P〈0.01）. CRP, WBC levels had no significant difference （F=7.77,F=5.49;P〉0.05） between Infection group and non-infection group. （2） PCT, CRP and WBC sensitivity for the diagnosis of infection was 93.18%（41/44）, 75.01%（33/44） and 43.18%（19/44）, PCT infection and CRP combined sensitivity of up to 97.73% （43/44）.Conclusion： In the diagnosis of Emergency observation infection, sensitivity and specificity of PCT were better than CRP and WBC, PCT and CRP test can improve the diagnosis when combined with emergency observation infected with sensitivity.