Notch信号通路在肝癌细胞迁移过程中的作用及机制

The mechanism of Notch signaling pathway in the migration of hepatocellular carcinoma cells

目的:探讨Notch信号通路在肝癌细胞迁移过程中的作用及相关机制。方法:体外培养肝癌细胞系SMMC-7721、MHCC97H和正常非肿瘤肝细胞系HL-7702,利用Transwell小室检测细胞的迁移能力,Western blot检测Notch1、Snail、E-cadherin、COX-2(环氧化酶-2)蛋白的表达。分别采用5μmol/L DAPT(3,5-二氟苯乙酰-L-丙氨酰-S苯基甘氨酸t-丁酯,γ-分泌酶抑制剂)阻断Notch信号通路和50μmol/L NS-398阻断COX-2,比较对细胞迁移能力的影响以及对肝癌细胞中Snail、E-cadherin表达的影响。结果:Notch1、Snail、E-cadherin、COX-2参与了肝癌细胞迁移过程,DAPT通过阻断Notch显著上调E-cadherin,影响细胞的迁移(P<0.05),下调Snail和COX-2,NS-398通过阻断COX-2影响细胞的迁移(P<0.05)。结论:Notch信号通路在肝癌细胞迁移过程中起着非常重要的作用,其机制初步认为与Snail、E-cadherin、COX-2的表达有关。

Objective：To explore the mechanism of Notch signaling pathway in the migration of hepatocellular car-cinoma cells. Methods：Cultured human hepatoma SMMC - 7721,MHCC97H and normal liver HL - 7702 cells in vitro,using transwell assay to test the ability of cell migration. Using Western blot to detect the protein expression of Notch1,Snail,E - cadherin and COX - 2. Compared the cell migration ability before and after blocking Notch signa-ling pathway with 5μmol/ L DAPT（γ secretase inhibitors）and blocking COX - 2 with 50μmol/ L NS - 398. Results：Notch1,Snail,E - cadherin and COX - 2 were involved in the migration process of HCC cells. The effect of DAPT could up - regulate the expression of E - cadherin and down - regulate the expression of Snail and COX - 2. Conclu-sion：The role of Notch signaling pathway in HCC cells migration is very important,and the mechanism maybe related to the levels of Snail,E - cadherin and COX - 2.