糖肾宁对自发性2型糖尿病KK-Ay小鼠肾组织血小板衍生生长因子及受体表达的影响

Effects of Tangshenning on PDGF,PDGFR expression of renal tissue in Type 2 diabetic KK-Ay Mice

目的观察糖肾宁对自发性2型糖尿病KK-Ay小鼠肾组织血小板衍生生长因子(platelet-derived growth factor,PDGF)、血小板衍生生长因子受体(platelet-derived growth factor receptor,PDGFR)蛋白表达的影响。方法采用自发性2型糖尿病KK-Ay小鼠建立糖尿病肾病模型,随机分为模型组、缬沙坦组和糖肾宁组各20只,设立C57BL/6J小鼠20只为空白组;药物干预12周后,测定24小时尿微量白蛋白排泄率(urinary albumin excretion,UAER),Masson染色观察各组肾脏病理,Western Blot检测PDGF、PDGFR蛋白表达水平。结果与空白组比较,模型组24小时UAER明显升高(P<0.05),肾组织PDGF、PDGFR蛋白表达明显升高;与模型组比较,缬沙坦组和糖肾宁组24小时UAER均有不同程度的降低(P<0.05),给药组间无显著性差异(P>0.05),肾组织PDGF、PDGFR蛋白表达有不同程度降低(P<0.05)。结论糖肾宁能够降低自发性2型糖尿病KK-Ay小鼠24小时UAER,抑制PDGF及PDGFR蛋白的过度表达,保护肾功能。

Objective The aim of this study is to explore the effects of Tangshenning on renal function and expressions of Platelet-derived Growth Factor （ PDGF ）、Platelet-derived Growth Factor Receptor （ PDGFR） on diabetic nephropathy mice model. Methods Spontaneous type 2 diabetic male KK -Ay mice were randomly divided into three groups：the model group （n=20）,the valsartan group（n=20） and the Tangshenning group （n=20）. Male C57BL/6J mice were chosen as control group （n=20）. After 12 weeks of treatment with Tangshenning and valsartan, 24h Urinary albumin excretion （24h UAER） was examined and the expressions of PDGF,PDGFR were assessed by Western Blot. We also observed the renal pathological changes by Masson staining. Results Compared to that of in the control group, 24h UAER and the expressions of PDGF and PDGFR in the model group are significantly increased. Both＆amp;nbsp;treatment groups had significantly lower 24h UAER （P〈0. 05） and PDGF and PDGFR protein expressions compared to the model group. Conclusion Tangshenning can protect renal function and decrease 24h UAER, which may due to the inhibition of PDGF and PDGFR protein expressions.