SPME-GC-MS检测尿液中甲卡西酮类毒品

Detection of methcathinones in urine samples by SPME-GC-MS

建立了尿液中甲卡西酮类毒品顶空固相微萃取-气相色谱-质谱联用的含量测定方法。在添加定量甲卡西酮类标准品的尿液试样中,加入固体Na2CO3,以MDMA为内标,调溶液p H10,80℃下加热搅拌,用70μm乙烯二醇-二乙烯基苯共聚物(CW/DVB)萃取头顶空萃取20 min,GC-MS检测,获得良好线性,其标准曲线线性范围在0.06μg^1.86μg·m L-1,最小检测限可达0.01μg·m L-1,平均回收率均在85%~115%之间。相对标准偏差均在11%以内。

A simple method for detection of methcathinones in urine by SPME-GC-MS（ SIM） was developed. In the urine sample,the standard solution of methcathinones,solid sodium carbonate and MDMA（internal standard solution）was added. The solution was stirred and heated under 80℃ on the condition of pH10. Then,the sample was extracted by HS-SPME with 70μm ethyleneglycol-vi-nylbenzene copolymer（ CW/DVB） ,a middle-polar coated fiber for 20 minutes and then analyzed by GC-MS. The result show good linearity in the range of 0. 06μg-1. 86μg·mL^-1 and LOD was 0. 01μg·mL^-1. The value of accuracy was varying from 85% -115% and RSD was within 11%.