蒺藜茎叶的化学成分研究

Study on chemical constituents in the stems and leaves of Tribulus terrestris

目的:研究蒺藜Tribulus terrestris L.茎叶的化学成分。方法:采用大孔树脂、MCI树脂、RP-C18键合硅胶柱色谱、Sephadex LH-20凝胶柱色谱、反相制备高效液相色谱等技术对蒺藜提取物进行分离纯化,并根据理化性质和波谱手段进行结构鉴定。结果:从蒺藜茎叶30%大孔树脂洗脱物中分离得到12个化合物,鉴定出10个化合物,分别为乙酰丁香酮-4’-O-β-D-吡喃葡萄糖苷(1),异嗪皮啶-7-O-β-D-吡喃葡萄糖苷(2),异鼠李素-3-O-β-D-葡萄糖基-7-O-β-D-吡喃葡萄糖苷(3),槲皮素-3-O-β-D-龙胆双糖苷(4),异荭草苷(5),山奈酚-3-O-β-D-龙胆双糖基-7-O-β-D-吡喃葡萄糖苷(6),异鼠李素-3-O-β-D-龙胆双糖基-7-O-β-D-吡喃葡萄糖苷(7),Terrestrinone A2(8),邻苯二甲酸二丁酯(9)和邻苯二甲酸二辛酯(10)。结论:化合物1和5为首次在该属中分离得到。

Objective： To investigate the chemical constituents in the stems and leaves of Tribulus terrestris. Methods： The compounds were isolated and purified by a combination of various column chromatographies techniques including macroporous resin, MCI resin, reversed-phase C18 silica gel, Sephadex LH-20 columns, and reversed-phase HPLC. The structures were identified by means of chemical evidences and spectral analyses including MS, 1H-NMR, and 13C-NMR. Results： Twelve compounds were isolated and 10 were identified as 3＇, 3＇-dimethoxy-4＇-hydroxyacetophenone-4＇-O-β-D-glucopyranoside （1）, isofraxidin-7-O-β-D-glucopyranoside （2）, isorhamnetin-3-O-β-D-glucosyl-7-O-β-D-glucoside （3）, quercetin-3-O-β-D- gentiobioside （4）, isoorientin （5）, kaempfero 1-3-O-β-D-gentiobioside-7-O-D-glucoside （6）, isorhamnetin-3-O-β-D-gentiobioside- 7-O-D-glucoside （7）, Terrestrinone A2 （8）, dibutyl phthalate （9）, dioctyl phthalate （10）. Conclusion： Compounds 1 and 5 are isolated from the plants of Tribulus for the first time.