岗藿抗感汤对小鼠H1N1流感病毒肺炎的影响

Effects of Ganghuo Kanggan Decoction on viral pneumonia mice caused by H1N1 influenza virus

目的:研究岗藿抗感汤对H1N1流感病毒FM1株感染引致的小鼠病毒性肺炎的影响。方法:采用流感病毒滴鼻感染正常小鼠建立小鼠H1N1流感病毒肺炎模型。观察岗藿抗感汤对小鼠肺泡灌洗液中白细胞总数及巨噬细胞、淋巴细胞、中性粒细胞所占比例,及其血清中白细胞介素6(IL-6)、白细胞介素10(IL-10)、白细胞介素12(IL-12)、干扰素γ(IFN-γ)、肿瘤坏死因子α(TNF-α)、趋化蛋白因子1(MCP-1)等炎性因子的影响。结果:岗藿抗感汤能显著减少H1N1流感病毒肺炎小鼠炎性介质的渗出;提高抗炎因子IL-10、IFN-γ水平,降低IL-6、TNF-α、MCP-1等促炎因子水平;降低肺泡灌洗液中白细胞总数,升高巨噬细胞百分比,降低中性粒细胞、淋巴细胞比例。结论:岗藿抗感汤可调节抗炎、促炎因子水平,减轻H1N1流感病毒引致的小鼠肺部炎性反应。

Objective： To study the influence of Ganghuo Kanggan Decoction to viral pneumonia mice caused by H1N1 influenza virus FAIl strain infected. Methods： The leukocytes and macrophages, lymphocytes, neutrophils proportion in murine alveolar lavage fluid were counted. Additionally, the concentration of interleukin-6 （IL-6）, interleukin-10 （IL-10）, interleukin-12 （IL-12）, interferon-7 （IFN-7）, tumor necrosis factor-α （TNF-α）, chemotactic of the protein factor 1 （MCP-1） in serum were assessed to viral pneumonia mice, which have been intranasally infected by Influenza A （H 1N 1） virus. Results： Ganghuo Kanggan Decoction could significantly reduce leakage of inflammatory mediators, improve anti-inflammatory cytokine IL-10, IFN-7 levels, lower IL-6, TNF-α, MCP-1 and other pro-inflammatory cytokines, and reduce the total number of leukocytes in bronchoalveolar lavage fluid, increase the percentage of macrophages, neutrophils decreased, the proportion of lymphocytes. Conclusion： Ganghuo Kanggan Decoction could adjust anti-inflammatory, pro-inflammatory cytokine levels and alleviate mouse lung inflammation caused by virus.