酮替芬抗炎症作用对糖尿病大鼠的影响

目的 探讨酮替芬（KT）抗炎症作用对糖尿病（DM）大鼠的影响及其机制.方法 以高糖高脂饲料对SD大鼠饮食诱导6周,随后一次性给予链脲佐菌素（STZ）制备DM大鼠模型,模型建立后KT实验组给予KT0.09mg/（kg·d）治疗8周,治疗前后分批处死实验大鼠,检测空腹血糖（FBG）、空腹胰岛素（FINS）水平,计算胰岛素敏感指数（ISI）、胰岛素抵抗指数（HOMA-IR）,检测甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-C）、炎症因子IL-6和TNF-α.结果 KT可降低FBG、FINS水平（P＜0.05）,降低TG、LDL-C水平（P＜0.05）,降低IL-6、TNF-α水平（P＜0.05）.结论 KT可能通过抑制肥大细胞脱颗粒、抑制NF-κ B激活,改善DM炎症及脂代谢紊乱状态,实现对胰岛细胞的保护作用.

Objective To investigate the effect and mechanism of the anti-inflammatory role of Ketotifen in diabetic rats induced by high- caloriediet and streptozocin. Methods Male SD rats were fed with high-carbon hydrate-fat diet 6 weeks and ip given STZ to in-duce a type 2 diab-etes model. Rats of Ketotifen group were fed with Ketotifen 0.09mg/（kgod）, and killed in batches in different time （ o, 8th week ） to test the fasting blood glucose （ FBG ） , and insulin （ FINS ） levels were measured, insulin sensitivity index （ ISI ） and homeostasis model assessment insulin resistance index （ HOMA-IR ） were calculated according to the value of FBG and FINS, to test the level of triglyceride （ TG ） , low density lipoprotein cholesterin （ LDL-C ） , Interleukin-6 （ IL-6 ） and tumor nectosis factor-alpha （ TNF- α） . Results Ketotifen suppressed serum glucose in diabetic rats （P〈0.05） , increase-d ISI and reduced HOMA-IR （P〈0.01） , lowered TG and LDL-C in diabetic rats （P〈0.05） , Ket-otifen reduced the level of IL-6, TNF-α. Conclusion Ketotifen can improve the flammatory and dyslipidaemia state of type 2 diabetic rats by inhibition of mast cell degranulation and NF- κB activation.