唑尼沙胺对戊四氮致痫小鼠神经元特异性烯醇化酶水平变化的影响

Study the Changes of the Levels of Neuron Specific Enolization Enzymes in Epilepsy Mice Induced by Pentetrazol after Zonisamide Treatment

目的:观察应用唑尼沙胺对致痫小鼠癫痫发作的疗效,通过检测血清神经元特异性烯醇化酶(NSE)水平变化,探讨唑尼沙胺对脑神经损伤的保护作用。方法:本研究利用戊四氮建立发育期小鼠癫痫模型,随机分为正常对照组、癫痫模型组、唑尼沙胺组,观察小鼠惊厥发作频率和发作程度,测定血清NSE水平及观察海马组织形态学的变化。结果:正常对照组无惊厥发作,血清NSE水平在正常范围;癫痫模型组惊厥出现时间早,发作程度重,血清NSE水平明显高于其他两组(P均<0.01);唑尼沙胺组惊厥出现时间晚,发作程度轻,血清NSE水平略高于正常对照组而明显低于癫痫模型组。结论:唑尼沙胺可减轻戊四氮致癫小鼠的癫痫发作时间和严重程度,降低血清NSE水平,提示唑尼沙胺对脑神经损伤具有保护作用,为癫痫患儿寻找一种有效的、广谱抗癫痫药物提供了实验室依据。

Objective： To study the efficacy of zonisamide to the epilepsy mice,and observe its protective effect to the brain damage through the changes of the levels of neuron specific enolization enzymes（ NSE）. Methods： Epilepsy mice models were induced by pentetrazol. The mice were randomly classified into three groups,as normal control group,model group and treatment group. The degree of convulsion and the seizure frequency were recorded everyday. The levels of serum NSE were measured by ELISA method and the morphological changes of hippocampus tissue were observed. Results： Convulsion did not attacked and the level of serum NSE was normal in normal control group. Convulsion attacked early and seriously in model group,the serum NSE level significantly was higher than the other two groups; Convulsion attacked latter and lighter in treatment group,the serum NSE level significantly was higher than the normal control group and lower than the modal group. Conclusion： The level of serum NSE is decreased after zonisamide treatment,and zonisamide has neuroprotective effects in the epilepsy mice model. We should look for a kind of effective,broad spectrum antiepileptic drugs for children with epilepsy to provide laboratory basis.