Survivin和CK19在肝细胞癌中的表达及意义

THE EXPRESSION OF SURVIVIN AND CK19 AND THEIR CLINICAL SIGNIFICANCE IN HEPATOCELLULAR CARCINOMA

目的研究survivin和CK19在肝细胞癌(Hepatocellular carcinoma HCC)中的表达及临床病理意义。方法采用组织芯片技术及免疫组化SP法,研究296例HCC和81例癌旁组织中survivin和CK19的表达,并探讨其与临床病理特征及预后的关系。结果 1survivin和CK19在HCC中表达的阳性率分别为72.3%(214/296)和14.5%(43/296),均高于癌旁组织的9.9%(8/81)和0(0/81),差异均有统计学意义(P均<0.05)。2Survivin在HCC中的表达与组织学分级、临床分期、术后无瘤生存时间、肿物直径、淋巴结转移及肝被膜浸润相关,差异具有统计学意义(P均<0.05),与其他指标无关(P均>0.05);CH19在HCC中的表达与肝硬化、组织学分级、临床分期和淋巴结转移相关,差异具有统计学意义(P均<0.05),与其他指标无关(P均>0.0)。3Kaplan-Meier生存分析显示,survivin和CK19在HCC中表达阳性的患者无论其术后无瘤生存时间还是其总生存时间均短于阴性表达患者,差异均具有统计学意义(P均<0.05)。4Pearson相关分析示,survivin和CK19在HCC中的阳性表达呈正相关(r=0.146,P=0.012)。结论 HCC中survivin和CK19的阳性表达与多种临床病理指标相关,两者均可作为HCC患者预后不良的判断因子。

Objective To study the expression of survivin and CK19 protein and their clinicopathologic significance in hepatocellular carcinoma （HCC） .Meth‐ods The expression of suevivin and CK19 were deter‐mined by tissue microarray and immunohistochemistry 〈br〉 in 296 cases of HCC and 81 cases of non‐cancerous tissue .T he relationships betw een their expression and the clinicopathologic parametersand ,the survival time of patients w ere analyzed .Results T he possive rates of survivin and CK19 in HCC were 72 .3% and 14 .5% ,which were higher than those in non‐cancerous tis‐sue（ P 〈0 .05） .The expression of survivin in HCC was closely correlated to the histological grade ,TNM clicinal stage ,survival time ,lymph node mestastasis ,tumor diameter and liver capsule invasion（ P 〈0 . 05） ,but not to the rest of parameters .The xpression of CK19 in HCC was closely correlated to the liver cirrhosis ,the histological grade ,TNM clicinal stage and lymph node mestastasis（ P 〈0 .05） ,but not to the rest of parameters .The positive expression of survivin and CK19 were significantly related to short‐term survival by Kaplan‐Meier analysis （ P〈0 .05） .The two markers were positively interrelated （ r =0 . 146 ,P =0 .012） .Conclusion The positice expression of survivin and CK19 closely correlates with multiple clinicopathologic parameters ,w hich may indicate the poor prognosis of HCC .