SLC26A9基因多态性与儿童哮喘相关性分析

Association between polymorphism of SLC26A9 gene and clinical characteristics of asthma in children

目的探讨我国中部地区汉族儿童中SLC26A9基因SNP位点(rs2282430和rs12031234)多态性与哮喘及其临床特点的关系。方法利用病例对照的研究方法,选取203例哮喘患儿和221例健康儿童为研究对象,利用限制性片段长度多态性分析(PCR-RFLP)的方法检测两个SNP位点多态性,并进行统计分析。结果哮喘患儿与对照组之间,SNP位点rs2282430的3种基因型(AA、AG、GG)分布差异有统计学意义(P=0.042),哮喘患儿AA基因型的比例较高;在隐性模式下(AA对AG+GG),两组相比差异有统计学意义(P=0.028)。哮喘患儿SNP位点rs2282430的A等位基因频率高于对照儿童,差异有统计学意义(P=0.011),提示A等位基因为风险因子。而哮喘患儿与对照组儿童之间,SNP位点rs12031234的3种基因型(TT、GT、GG)分布差异无统计学意义(P=0.479),等位基因频率差异也无统计学意义(P=0.215)。哮喘患儿SNP位点rs2282430不同基因型之间,淋巴细胞总数、中性粒细胞百分比、C反应蛋白、免疫球蛋白E、以及嗜酸性粒细胞百分比的差异均无统计学意义(P>0.05)。结论 SLC26A9基因SNP位点rs2282430的多态性与我国中部地区儿童哮喘的易感性相关;但该位点的多态性与LYM、CRP、Ig E、NEU%以及EOS%等指标不具有相关性。

ObjectiveTo investigate the association between nucleotide polymorphisms （SNP） of rs2282430 and rs2031234 inSLC26A9 gene and clinical characteristics of asthma in Han children in central China.MethodsA case-control study was performed. Two hundreds and three children with asthma were recruited in this study and 221 normal children were selected as controls. The genotypes of two SNPs inSLC26A9 gene were examined using PCR-RFLP.ResultsBetween children with asthma and controls, the distribution of three genotypes （AA, AG and GG） in rs2282430 locus had signiifcant difference （P=0.042）. The percentage of AA genotype was higher in children with asthma than that in controls. In implicit mode （AAvs. AG＋GG）, the two groups was statistically signiifcant difference （P=0.028）. The frequency of A allele was higher in children with asthma than that in controls （P=0.011）. Between children with asthma and controls, the distribution of three genotypes （TT, GT, and GG） in rs12031234 locus had no signiifcant difference （P=0.479）. The frequency of alleles in rs12031234 locus also had no signiifcant difference （P=0.215）. Among asthmatic children with different genotype of rs2282430, the lymphocytecounts （LYM）, C-reaction protein （CRP）, IgE, neutrophils （NEU%）, and eosinophils （EOS%） were not signiifcantly different （P〉0.05）. Conclu-sionsThe rs2282430 polymorphism inSLC26A9 gene is associated with childhood asthma in the central China and the A allele is the risk factor. The rs2282430 polymorphism is not associated with LYM counts, CRP level, IgE level, NEU%, and EOS%.