虎杖煎剂对急性肺损伤大鼠TNF-a,IL-1β表达的影响

Effect of Polygonum Cuspidatum on Expression of TNF-α and IL-1β in Acute Lung Injury Albino Rats

目的探讨虎杖(Polygonum Cuspidatum,PC)煎剂对细菌脂多糖(lipopolysaccharide,LPS)诱导Wistar大鼠急性肺损伤(acute lung injury,ALI)的肿瘤坏死因子-α(TNF-α)、白介素1β(IL-1β)表达的影响,探寻虎杖煎剂治疗急性肺损伤的作用机制。方法 60只Wistar大鼠随机分成6组,每组10只,分别为空白对照组,模型对照组,激素对照组,虎杖低、中、高剂量组。造模前24h、12h及造模后12h、24h、36h分别对空白对照组和模型对照组予0.9%生理盐水8m L/kg灌胃1次,激素对照组予强的松6.25mg/kg灌胃1次,虎杖低、中、高剂量组分别予虎杖煎剂2.6g/kg、5.2g/kg、13g/kg灌胃1次。造模48h后处死大鼠,收集样本,进行测定和病理切片检查。用ELISA法检测ALI大鼠肺泡灌洗液及肺组织中TNF-α、IL-1β。结果虎杖低、中、高剂量组肺泡灌液中TNF-α浓度分别为(112.6±3.2)pg/m L、(110.9±4.0)pg/m L、(108.9±3.4)pg/m L,IL-1β浓度为(122.4±3.9)pg/m L、(118.7±2.8)pg/m L、(117.2±2.3)pg/m L,与模型对照组相比,除虎杖低剂量组IL-1β浓度无明显变化(P>0.05)以外,其余均显著降低(P<0.01或P<0.05);虎杖低、中、高剂量组肺组织中TNF-α浓度分别为(81.9±3.9)pg/m L、(77.3±6.4)pg/m L、(76.6±2.9)pg/m L,IL-1β浓度分别为(66.7±6.1)pg/m L、(65.1±1.9)pg/m L、(62.8±3.8)pg/m L,与模型对照组相比,除虎杖低、中剂量组IL-1β浓度无明显变化(P>0.05)外,均显著降低(P<0.01或P<0.05)。结论虎杖煎剂可抑制炎症细胞因子TNF-α、IL-1β的产生,减轻肺损伤程度,这可能是虎杖煎剂治疗急性肺损伤作用机制之一。

Objective To observe the influence of the Polygonum Cuspidatum（PC） decoction on Acute Lung Injury（ALI） of TNF-α, IL-1 beta expression in LPS induced Wistar rat, and to explore The PC decoction for the treatment of Acute Lung Injury. Methods 60 Wistar rats randomly divided into 6 groups, each group of 10, respectively, blank control group, model control group, hormone control, PC low, medium and high dose group. Building before 12 h and 24 h, 12 h, 24 h and after building 36 h respectively to the blank control group and model control group to 0.9% saline 8 ml/kg one time to fill the stomach; Hormone control group to prednisone 6.25 mg/kg lavage. PC low, medium and high dose group respectively for the giant knotweed decoction of 2.6 g/kg, 5.2 g/kg, 13 1 g/kg to fill the stomach by the way. Building to be put to death in the rat after 48 h, collecting samples, measured and pathological biopsy. With ELISA method to detect ALI rats bronchoalveolar lavage fluid （BALF） and lung tissue of TNF-α, IL-1β. Results PC low, medium and high dose group of concentration of TNF-α are （112.6±3.2）pg/mL, （110.9±4.0）pg/mL, （108.9±3.4）pg/mL, IL-1±concentration for（122.4±3.9）pg/mL, （118.7±2.8）pg/mL, （117.2±2.3）pg/mL, compared with model control group, except for the PC low dose group of IL-1±concentration has no obvious change （P〉0.05）, the rest were significantly lower（P〈0.01 or P〈0.05） in BALF; PC low, medium and high dose group of TNF-αconcentration in the lung tissue（81.9±3.9）pg/mL, （ 77.3 ±6.4 ）pg/mL, （ 76.6±2.9 ）pg/mL, IL- 1βconcentration respectively （ 66.7±6.1 ）pg/mL, （ 65.1 ± 1.9 ）pg/mL, （ 62.8 ±3.8 ）pg/mL, compared with model control group, except for the PC low dose group of IL-1βconcentration has no obvious change （P〉0.05）, were significantly lower（P〈0.01 or P〈0.05）. Conclusion The PC decoction can inhibit the production of the inflammatory cytokine TNF-α and IL-1β, it could be a PC mechanism of decoction in treatment of acute lung injury.