高脂血症血清脂肪酸分析

Research on biomarkers in serum fatty acids of hyperlipidemia

探讨高脂血症小鼠血清脂肪酸水平的变化。以高脂血症小鼠为模型,以生化检测指标为基础,通过GC—MS检测技术监测基础组、高脂组及配方油脂干预组小鼠血清脂肪酸的差异。将50只SPF级KM系雄性小鼠随机分为基础组、高脂组、配方油低中高剂量组。连续喂养4周,提取小鼠血清,检测血清生化指标,并使用气相色谱—质谱联用技术检测其脂肪酸的种类与含量。结果表明,成功建立高脂血症小鼠模型,且小鼠血清脂肪酸种类并无差别;而与基础组比较,高脂组C14∶0、C16∶1、C18∶1、C22∶6的含量有显著性差异;与高脂组相比,配方油组各脂肪酸含量均有不同程度变化,其中C16∶1、C18∶1、C22∶6的含量变化趋势显著(P<0.05);且不饱和脂肪酸比例在各组之间也有显著性差异。根据GC—MS(气质联用)监测结果可知,C16∶1、C18∶1、C22∶6三种脂肪酸在各组之间变化显著,与高脂血症代谢异常有关,血清不饱和脂肪酸比例与高脂血症关系密切。

To explore the differences of serum free fatty acids（ FFA） of hyperlipidemia. Based on the hyperlipidemia model,the biochemical detection index,looking at the differences of serum fatty acids of group,fat group,formula oil low,medium,high dose groups. After continuously feeding four weeks,the serum was taken,the biochemical indexes were detected and the content of fatty acids were detected with gas chromatography-mass spectrometry. The results showed that the model of hyperlipidemia was successfully established and there was no species difference in all these fatty acids. Compared with the basic group,the content of C14∶ 0,C16∶ 1,C18∶ 1,C22∶ 6 of high-fat group were significantly different. Compared with the high-fat group,the fatty acid of formula oil groups varied on some degrees,and the diversification of C16∶ 1、C18∶ 1、C22∶ 6 was significantly. The ratio of unsaturated fatty acid was significantly difference between the groups. Based on GC-MS monitoring results,we could know that C16∶ 1,C18∶1,C22∶ 6 changed significantly between the groups,and could be related with the abnormal metabolism of hyperlipi-demia,and the proportion of unsaturated fatty acids in serum had a close relationship hyperlipidemia.