Akt信号在苯丙胺致大鼠黑质损伤中的变化

Change of Akt pathway in amphetamine-induced injury in rat nigra substantia

目的探讨Akt信号在苯丙胺致大鼠黑质损伤中的变化。方法给大鼠腹腔注射苯丙胺(2.5 mg·kg-1·d-1),分为苯丙胺1 h、1 d、7 d和14 d组。用透射电镜观察大鼠黑质超微结构的变化,用Western blot检测Akt和m TORC2蛋白在黑质的变化。结果苯丙胺14 d组一些黑质神经元结构模糊,核固缩。许多髓鞘变形,髓鞘板层不清晰。苯丙胺1 h P-Akt(Thr308)表达没有显著性变化。苯丙胺1 d组、7 d组和14 d组的P-Akt(Thr308)表达明显减少。苯丙胺各组P-rictor(S1219)的表达均无显著性差异。结论苯丙胺对大鼠黑质神经元的损伤可能与抑制Akt信号有关。

Objective To explore the change of Akt pathway in amphetamine-induced injury in rat nigra substantia.Methods The rats was given d-amphetamine（2.5 mg·kg^-1·d^-1） by intrapertoneal injection. The amphetamine model group was subdivided into 1 h, 1 d, 7 d and 14 d subgroups. The ultrastrucutures of the nigra sunstantia were observed by transmission electron microscope. The levels of Akt and m TORC2 were measured by Western blot. Results After amphetamine administration 14 days,some neuronal ultrastractures were obscure, and had condensation nuclei. And many myelinated nerve fibers were distorted. Laminae of some myelin sheaths were not clear in the nigra substantia. The level of p-AKT（Thr308） was not altered after amphetamine administration 1 h. However, p-AKT（Thr308） level was decreased in the nigra substantia at 1 day, 7 days and 14 days after amphetamine. Amphetamine did not alter the level of p-rictor（Ser1219） in the nigra substantia. Conclusion The inhibition of Akt signaling pathway may contribute to damage of the nigra substantia induced by amphetamine.