小麦PAL基因的克隆及赤霉菌诱导下的表达分析

Cloning of PAL Genes and Their Response to FHB in Wheat

利用苯丙氨酸解氨酶(PAL,phenylalanine ammonia-lyase)基因保守区域从小麦抗赤霉病材料苏麦3号中克隆获得4个PAL基因,分别命名为Ta PAL1、Ta PAL2、Ta PAL3、Ta PAL4。4个基因的开放阅读框(ORF,open reading frame)长度分别为2142 bp、2016 bp、2118 bp和2139 bp,分别编码714个、672个、706个和713个氨基酸。基因序列比对发现其相似性达到88.35%,所编码的氨基酸相似性为91.92%,氨基酸序列分析表明4个基因都包含HAL-PAL结构域及PAL结构域。通过接种禾谷镰刀菌,利用荧光定量PCR对PAL基因进行表达分析发现,4个PAL基因全部为上调表达,其中Ta PAL2、Ta PAL3和Ta PAL4最为明显。PAL基因的上调表达,说明PAL基因在小麦抵抗赤霉病菌侵染的机制中可能起着重要作用。

Four PAL（ phenylalanine ammonia-lyase）genes were cloned from Sumai3 ,which were designed as TaPAL1, TaPAL2, TaPAL3 and TaPAL4. Their Open reading frames （ ORFs ） were 2142 bp, 2016 bp, 2118 bp and 2139 bp, and the length of the deduced protein were 714,672,706 and 713 amino acid residues, respectively. Se- quence alignment of the four genes showed the similarity was 88.35% and the encoded amino acids was 91.92%. Amino acids sequence analysis showed that the four proteins all contained HAL-PAL domain and PAL domain. Expression pattern analysis revealed that all the four PAL genes were up regulated after inoculation with Fusarium graminearum, which suggested that PAL genes might have an important role in the defense response to F. graminearum.