胡萝卜中DcDofD1转录因子的克隆及其对非生物逆境胁迫的响应分析

Cloning and Expression Analysis of a Transcription Factor,DcDofD1 Related to Abiotic Stress Reaction in Carrot

Dof(DNA-binding with one finger)转录因子是植物特有的一类转录因子,该类转录因子在植物生长发育过程中起重要作用。本试验以胡萝卜黑田五寸和君川红为试验材料,分别从中克隆获得Dc Dof D1转录因子基因。采用生物信息学方法对Dc Dof D1转录因子氨基酸组成、理化性质、进化关系进行了分析。并利用实时定量PCR方法对Dc Dof D1基因在非生物胁迫下的表达量进行了分析。结果表明,黑田五寸和君川红中的Dc Dof D1转录因子具有明显的单锌指结构,保守域的氨基酸序列比较保守。进化分析显示,Dc Dof D1属于Dof家族的D1亚族。在胡萝卜黑田五寸和君川红中,Dc Dof D1基因对高温、低温、干旱、盐等非生物胁迫有响应。而不同胡萝卜材料中,Dc Dof D1基因对非生物逆境响应的强度和速度不同。

In higher plant, Dof（ DNA-binding with one finger）transcription factor is a plant-specific transcrip- tion factor, which plays important roles during the growth and development. Here, the genes of DcDofD I , which en- coding to a Dof transcription factor, were cloned from two carrot varieties ＇ Heitianwucun＇ and ＇ Junchuanho,hg＇ , re- spectively. The sequences of nucleic acid and amino acid, phylogenetic tree, hydrophilicity and hydrophobioity, and molecular modeling were analyzed using bioinformaties method. The results demonstrated that the two DcDofD1 fac- tors from carrot had single zinc finger, and their amino acid sequences at Dof domain were very conserved. The DcD- of D1 factors from carrot were classified into D1 subfamily of Dof family. The expression profiles of the DcDofDl gene under four abiotic stress treatments （cold, heat, high salt, and drought）were analyzed by quantitative real-time PCR in the two carrot varieties ＇ Heitianwucun＇ and ＇ Junchuanhong＇. The results of expression profiles showed that DcDofD 1 gene responded to the cold, heat, high salt, and drought stresses in carrot. The results demonstrated that the DcDofD I gene involved into interaction with abiotic stress in carrot.