骨髓基质干细胞体外定向诱导后表面MHC抗原的表达

MHC antigen expression on the surface of bone marrow stromal stem cells after directional induction in vitro

背景:同种异体骨髓基质干细胞移植治疗骨骼疾病是研究热点,寻找有效的方法防止移植后免疫排斥反应的发生,是当前迫切需要解决的问题。目的:探讨骨髓基质干细胞在体外诱导为成骨细胞后表面MHC抗原的表达。方法:抽取兔骨髓组织,在体外分离、培养获得骨髓基质干细胞,加入含骨形态发生蛋白2重组蛋白的IMDM诱导培养基诱导后,流式细胞仪分析骨髓源性成骨细胞MHC抗原的表达。结果与结论:骨髓源性成骨细胞表面MHCⅡ类抗原不表达、MHCⅠ类抗原表达。骨髓基质干细胞体外分化为成骨细胞后,未见免疫排斥反应,可用于同种异体或异种骨髓基质干细胞移植治疗骨缺损。

BACKGROUND：Alogeneic bone marrow stromal stem cel transplantation for treatment of bone diseases is a hot topic. To seek effective methods for prevention of post-transplantation immune rejection is urgent to be solved. OBJECTIVE：To explore the expression of MHC antigen on the surface of bone marrow stromal stem cels after osteogenic inductionin vitro. METHODS：Bone marrow samples were extracted from rabbits to in vitro isolate and culture bone marrow stromal stem cels. Then, the cels were cultured in IMDM medium containing bone morphogenetic protein-2. Flow cytometry was used to analyze the expression of MHC antigen on the osteoblasts differentiated from bone marrow stromal stem cels. RESULTS AND CONCLUSION：There was highly expressed MHC I antigen but no MHCII antigen on the osteoblasts differentiated from bone marrow stromal stem cels. After osteogenic induction, no immune rejection was found. These findings indicate that alogeneic or xenogeneic bone marrow stromal cel transplantation can be used in the treatment of bone defects.