骨性关节炎关节液中白细胞介素6和基质金属蛋白酶3的表达

Expression of interleukin-6 and matrix metalloproteinase-3 in the synovial fluid of osteoarthritis patients

背景:已有实验证实膝骨关节炎的发病与炎症细胞因子有密切的关系。目的:研究骨性关节炎患者关节液中白细胞介素6和基质金属蛋白酶3水平及其相关性,以及两者与骨性关节炎严重程度之间的相关性。方法:收集关节穿刺治疗的骨性关节炎患者关节液标本78份,应用ELISA法检测标本中白细胞介素6和基质金属蛋白酶3质量浓度,并对患者骨性关节炎严重程度进行HSS评分,采用Pearson相关分析探讨白细胞介素6、基质金属蛋白酶3和美国纽约特种外科医院(HSS)评分两两之间的相关性。结果与结论:骨性关节炎患者膝关节液中白细胞介素6为(13.1±5.7)μg/L,基质金属蛋白酶3为(989.3±429.5)μg/L,HSS为(56.4±12.0)分。Pearson相关分析显示白细胞介素6、基质金属蛋白酶3的表达水平呈显著性正相关关系(r=0.70,P<0.001);白细胞介素6和基质金属蛋白酶3均与关节美国纽约特种外科医院(HSS)评分均呈负相关关系(r=-0.70,r=-0.75,P<0.001)。结果显性关节液中白细胞介素6和基质金属蛋白酶3水平之间,以及两者与骨性关节炎严重程度之间均呈正相关,提示测定骨性关节炎患者关节液中白细胞介素6和基质金属蛋白酶3水平对早期诊断和治疗具有重要意义。

BACKGROUND： It is confirmed that the onset of osteoarthritis is closely related to inflammatory cytokines. OBJECTIVE： To investigate the levels of interleukin-6 and matrix metalloproteinase-3 in the synovial fluid of patients with osteoarthritis and their correlation with the severity of osteoarthritis. METHODS： Synovial fluid specimens of 78 patients were harvested to detect the levels of interleukin-6 and matrix metalloproteinase-3 using ELISA assay. The severity of osteoarthritis was estimated by the Hospital for Special Surgery （HSS） score. Pearson correlation analysis was adopted to explore the pairwise correlations between interleukin-6, matrix metalloproteinase-3 and HSS score. RESULTS AND CONCLUSION： The levels of interleukin-6 and matrix metalloproteinase-3 in the synovial fluid were （13.1±5.7） and （989.3±429.5） μg/L, respectively; the HSS score was （56.4±12.0） points. Pearson correlation analysis showed that the expression levels of interleukin-6 and matrix metalloproteinase-3 had a significant positive correlation （r=0.70, P 〈 0.001 ）; the levels of interleukin-6 and matrix metalloproteinase-3 had a negative correlation with the HSS scores （r= 0.70, r= 0.75, P 〈 0.001）. Experimental findings show that there is a positive correlation between interleukin-6 and matrix metallopreteinase-3 in the synovial fluids, and these two cytokines also have a positive correlation with the severity of osteoarthritis, indicating that to measure interleukin-6 and matrix metalloproteinase-3 levels in the synovial fluids of osteoarthritis patients is important for early diagnosis and treatment of osteoarthritis.