腰椎髓核组织中缺氧诱导因子1α的表达:与凋亡率正相关

Expression of hypoxia-inducible factor 1 alpha and its relationship to apoptosis in human lumbar nucleus pulposus

背景:缺氧诱导因子1α在缺氧的环境下对细胞凋亡起着双重调控作用。缺氧的严重程度决定细胞是出现凋亡还是适应缺氧生存。当细胞暴露于慢性或极度缺氧时由缺氧诱导因子1α引起的保护机制不足而发生凋亡。目的:观察缺氧诱导因子1α和凋亡在人不同突出类型腰椎髓核组织中的表达,判断两者有无相关性。方法:腰椎髓核组织标本取自腰椎间盘突出症行腰椎后路椎板开窗髓核摘除患者60例,41例取自L4-5髓核组织,19例取自L5-S1髓核组织。将髓核组织分为突出组、游离组各30例,同时选取腰椎骨折脱位患者腰椎髓核组织标本10例作为对照组。采用免疫组织化学技术,观察各组突出腰椎髓核组织缺氧诱导因子1α的表达;采用Tunel技术,观察不同突出类型腰椎髓核细胞凋亡程度;分析各组髓核组织缺氧诱导因子1α的表达与髓核细胞凋亡率有无相关性。结果与结论:游离组、突出组和对照组都可观察到缺氧诱导因子1α的表达,游离组缺氧诱导因子1α在髓核的表达显著高于突出组和对照组(P<0.01)。3组都可观察到髓核细胞的凋亡,游离组髓核细胞凋亡的表达显著高于突出型和对照组(P<0.01)。髓核细胞缺氧诱导因子1α表达和凋亡呈正相关(P<0.01)。结果提示,腰椎间盘突出症髓核组织中缺氧诱导因子1α的表达与突出类型有关,在脱垂游离型中表达最高。腰椎髓核组织中缺氧诱导因子1α的表达与凋亡率呈正相关。

BACKGROUND： Under hypoxic environment, hypoxia-inducible factor 1α plays a dual regulatory role in cell apoptosis. Severity of hypoxia is the key to determine whether cells appear to have apoptosis or adapt to survive. When the cells are exposed to chronic or extreme hypoxia, a lack of protection mechanisms from hypoxia-inducible factor-1α can induce cell apoptosis. OBJECTIVE： To research the expression of hypoxia-inducible factor la in human lumbar nucleus pulposus of different herniated types and its relationships with cell apoptosJs. METHODS： The nucleus pulposus was harvested from 60 cases of herniation of lumbar intervertebral discs, L4-5 in 41 cases and L5-S1 in 19 cases. The nucleus pulposus tissues were equally divided into protruded and sequestered groups. Meanwhile, the nucleus pulposus tissues from another 10 cases of lumbar spine fracture were taken as control group. Expression of hypoxia-inducible factor 1α and apoptosis of lumbar nucleus pulposus cells were observed and detected with immunohistochemical technique and TUNEL method. Correlation of hypoxia-inducible factor 1α and apoptosis in human lumbar nucleus pulposus of different herniated types was analyzed.RESULTS AND CONCLUSION： The expression of hypoxia-inducible factor la was visualized in each case, but it was significantly higher in the sequestered group than in the protruded group and control group （P 〈 0.01 ）, Apoptosis of nucleus pulposus cells were found in all the three groups, but the apoptotic rate was also higher in the sequestered group than in the protruded group and control group （P 〈 0.01）. Expression of hypoxia-inducible factor 1α] was positively correlated with cell apoptosis in human lumbar nucleus pulposus （P 〈 0.01 ）. Overall, the expression of hypoxia-inducible factor 1α in degenerative human lumbar nucleus pulposus is associated with herniated types, which is the highest in the sequestered type. The relationship between hypoxia-inducible factor 1α and apoptosis is positive.