荷移分光光度法测定交沙霉素片含量

Spectrophotometric Determination of Josamycin Tablets by the Charge Transfer Reaction

在乙醇介质中,交沙霉素与百里酚蓝发生荷移反应,以试剂为参比,体系在440 nm处产生一个新的吸收峰。在最佳反应条件下,交沙霉素在0.0032~0.036 mg/m L范围内,A440nm与ρ之间存在较好的线性关系,回归方程为A440nm=19.246ρ（mg/m L）-0.031,相关系数为0.9993,检出限为0.0021 mg/m L。该法用于测定交沙霉素片中交沙霉素的测定,具有简单、快速,准确的特点,适用于交沙霉素片的质量控制。

A novel method for the determination ofjosamycin by the charge transfer reaction between josamycin and thymol blue in ethanol. The experiments indicated that there is a maximum absorption peak at 440 nm by the charge transfer reaction between josamycin and thymol blue. Under the optimum condition, a linear relationship between the concentration of josamycin and A440nm is observed in the range of 0.0032-4）.036 mg/mL. The linear equation is A440nm -19.246 p（mg/mL）-0.031, relation coefficient is 0.9993 and detection limit is 0.0021 mg/mL. The proposed method is simple, fast, accurate, reliable and suitable for quality control ofjosamycin tablet.