辣椒多糖的提取及组成、分子量分布的测定

Extraction technique of capsicum annuum polysaccharides and determination of polysaccharides' content and molecular weight distribution

目的:以辣椒渣为原料,经过提取精制得到辣椒多糖,并研究了其单糖组成,分子量分布。方法:辣椒多糖采用水提醇沉法,经过酶法结合Savage法脱出蛋白,H2O2脱色法去除色素等分离精制手段对辣椒多糖进行了分离和精制。辣椒多糖经过水解、衍生化,通过GC测定其单糖的组成及含量,红外光谱研究辣椒多糖的结构特征,超滤法测定其分子质量分布。结果:通过提取分离得到的辣椒多糖,含量为86.5%,提取率为2.4%。由半乳糖、阿拉伯糖等7种单糖组成,均重分子量主要分布在10 KDa^50 KDa,占74.5%。辣椒多糖与枸杞多糖单糖组成较为相似,辣椒多糖比枸杞多糖多一个核糖的单糖组成。

Objective ： To obtain Capsicum annuum polysacchrides from Capsicum annuum residues and determination and fractions of polysacchrides content along with molecular weight distribution. Methods ： The yellowish Capsicum annuum powder were extracted from Capsicum annuum residues with hot water extraction and ethanol deposit, then futher deproteinize by means of Savage and enzyme. In addition, Capsicum annuum polysaccharides were decolorization which combined with H2O2, the content and composition of monosacchride were determined sequentially by acid hydrolysis, acetylation, and the structural characterization was carried out by infrared spectroscopy （FT-IR）, GC and molecular mass distribution of them were investigated by ultrafiltration. Results ： The Capsicum annuum polysacchrides were obtained with purity of 86.5 % and yield of 2.4 %, which were composed of 7 monosaccharides including galactose, ribnose, arabinose, glucose, xylose, rhamnse, mannose, and the molecular weights of most Capsicum annuum polysacchrides （74.5%） are from 10 KDa to 50 KDa. Results have shown that Lycium barbarum poiysacchrides and Capsicum annuum polysacchrides are similar in the composition of monosaccharide and the difference the two lies is ribnose.