西兰花提取物萝卜硫素抑制胃癌和胰腺癌的生长与侵袭

Sulforaphane Derived from Broccoli Inhibit Proliferation and Invasion of Gastric Cancer and Pancreatic Cancer

目的:观察萝卜硫素对胃癌和胰腺癌细胞活力、侵袭能力、周期、凋亡、DNA片段和相关蛋白的影响,为日常饮食提供参考,为临床治疗胃癌和胰腺癌提供实验数据。方法:通过CCK-8和transwell侵袭实验分析初步判断萝卜硫素对SGC-7901胃癌细胞和PANC-1胰腺癌细胞活性和转移侵袭的影响,计算体外干预SGC-7901和PANC-1的IC50,流式细胞学分析IC50浓度萝卜硫素对细胞周期和凋亡的影响,电泳分析DNA片段化改变。免疫印迹与免疫组化实验观察炎症和转移相关蛋白水平变化。结果:CCK-8结果显示SGC-7901和PANC-1细胞活性对萝卜硫素剂量依赖性下降,萝卜硫素作用于SGC-7901和PANC-1细胞的IC50分别为4.5μg/m L和5.5μg/m L(24 h),该浓度的萝卜硫素抑制了SGC-7901和PANC-1细胞的转移侵袭,阻滞细胞于G0/G1期,诱导细胞凋亡并使DNA发生片段化变化,下调TNF-α、TGF-β和NF-κB的表达。结论:萝卜硫素可以抑制胃癌和胰腺癌细胞的生长和侵袭,抑制炎症蛋白的表达,是胃癌和胰腺癌辅助治疗的新途径。

Objective:To investigate the effect of vitality, invasion, cell cycle, apoptosis, DNA fragment and relative proteins expression in gastric cancer SGC-7901 and pancreatic cancer PANC-1 cell line by sulforaphane(SFN)derived from broccoli, to provide reference for daily diet and supply experiment data for treatment gastric cancer and pancreatic cancer. Methods:Analyzed vitality and invasion of SGC-7901 and PANC-1 cells treated with sulforaphane by cell counting kit (CCK8) and transwell, then measure the half maximal (50%) inhibitory concentration (IC50) of sulforaphane for SGC-7901 and PANC-1 cells. The cells cycle, apoptosis and DNA fragment were analyzed using Flow Cytometry Analysis and agarose electrophoresis, TNF-α, TGF-β and NF-κB were analyzed by western blot and immunohistochemistry after treatment with sulforaphane. Results:Results showed that SGC-7901 and PANC-1 cells proliferate and invade were inhibited by sulforaphane with a dose-dependent manner, IC50 of sulforaphane was 4.5μg/mL(SGC-7901,24h) and 5.5μg/mL (PANC-1,24h), and the cell cycle were arrested at G0/G1 phase. 4.5μg/mL and 5.5μg/mL sulforaphane induced apoptosis, DNA fragment, decreased the expression of TNF-α, TGF-β and NF-κB in SGC-7901 and PANC-1 cells. Conclusion: Sulforaphane inhibited proliferation and invasion of gastric cancer SGC-7901 and pancreatic cancer PANC-1 cells in vitro, decreased the expression of inflammation proteins, maybe a novel chemotherapy for gastric cancer and pancreatic cancer.