维药蜀葵花化学成分的分离与鉴定(Ⅱ)

Isolation and identification of chemical constituents from Althaea rosea( Linn. ) Cavan.(Ⅱ)

目的对维药蜀葵花(Althaea rosea(Linn.)Cavan.)的化学成分进行进一步的研究。方法采用正相硅胶、反相ODS、Sephadex LH-20等柱色谱及高效液相色谱手段进行分离纯化,并通过理化性质与波谱分析方法鉴定化合物的化学结构。结果从蜀葵花体积分数为95%的乙醇提取物中分离得到9个黄酮类单体成分,分别鉴定为槲皮素(quercetin,1)、槲皮素-3-O-β-D-吡喃葡萄糖苷(quercetin-3-O-β-D-glucopyranoside,2)、槲皮素-3-O-(6″-O-反式对香豆酰基)-β-D-吡喃葡萄糖苷(quercetin-3-O-(6″-O-trans-p-coumaroyl)-β-D-glucopyranoside,3)、槲皮素-3-O-芸香糖苷(quercetin 3-O-rutinoside,4)、槲皮素-7-O-β-D-吡喃葡萄糖苷(quercetin-7-O-β-D-glucopyranoside,5)、槲皮素-4'-O-β-D-吡喃葡萄糖苷(quercetin 4'-O-β-D-glucopyranoside,6)、槲皮素-3'-甲氧基-3-O-芸香糖苷(quercetin 3'-methoxy-3-O-β-D-rutinoside,7)、杨梅素-3-O-β-D-吡喃葡萄糖苷(myricetin-3-O-β-D-glucopyranoside,8)和芹菜素-4'-O-β-D-吡喃葡萄糖苷(apigenin-4'-O-β-D-glucopyranoside,9)。结论化合物3、7、9为首次从蜀葵属中分离得到,化合物6为首次从蜀葵花中分离得到。

Objective To continue the study on the chemical constituents from Althaea rosea. Methods The compounds were isolated by using silica gel,ODS,Sephadex LH-20 and HPLC column chromatographies,and identified by the physico-chemical and spectroscopic methods. Results Nine flavonoids compounds were isolated and identified as quercetin（ 1）, quercetin-3-O-β-D-glucopyranoside（ 2）, quercetin-3-O-（ 6″-O-trans-p-coumaroyl）-β-D-glucopyranoside（ 3）,quercetin 3-O-rutinoside（ 4）,quercetin-7-O-β-Dglucopyranoside（ 5）, quercetin 4＇-O-β-D-glucopyranoside（ 6）, quercetin 3＇-methoxy-3-O-β-D-rutinoside（ 7）,myricetin-3-O-β-D-glucopyranoside（ 8）,and apigenin-4＇-O-β-D-glucopyranoside（ 9）. Conclusions Among the nine compounds,compounds 3,7,and 9 are obtained from Althaea genus for the first time,and compound 6 is isolated from this species firstly.