动物肠道乳酸菌的分离鉴定技术研究进展

Progress on Technologies of Isolation and Identification of Lactic Acid Bacteria in Animal Intestines

乳酸菌是可发酵碳水化合物产生大量乳酸的细菌的统称,是对人和畜禽有益的益生菌。动物肠道中的乳酸菌耐受胃酸有更好的适应性,更易于在肠道中定植抵抗病原微生物的黏附,并且耐酸性好易于生存。论文综述了动物肠道乳酸菌分离培养的几种方法,总结出更适合分离乳酸菌的培养基。归纳了动物肠道乳酸菌的鉴定方法,包括形态鉴定、生化鉴定以及分子生物学的鉴定,重点介绍了16SrDNA的分子生物学鉴定方法。这对建立动物肠道中乳酸菌分离鉴定的方法,研究动物肠道中的微生物菌群组成有重要意义。

The bacteria which can ferment carbohydrates to produce large amounts of lactic acid are called as lactic acid bacteria.They are probiotics beneficial to people and livestock and poultry.Lactic acid bacteria of animal intestines have a better adaptability because it has resistance to the acid.It could survive easily in the gut to resist pathogenic microorganism adhesion.This paper reviewed several methods of isolation and cultivation of lactic acid bacteria of animal intestines and summarized the better media to isolate lactic acid bacteria.This report summarized the methods to identificate actic acid bacteria in the intestines of animals, including morphological identification,biochemical identification and molecular biological identification methods,and focused on the 16 S rDNA of molecular biological identification.It has important significance to establish the methods for isolation and identification of lactic acid bacteria in the animal intestines and study the composition of microbial flora in the intestines of animals.