新生大鼠心肌telocytes在体外培养状态下的分裂方式

Division mode of cultured rat cardiac telocytes

目的探讨体外培养新生大鼠心肌telocytes的分裂方式。方法采用0.1%胶原酶I/0.125%胰蛋白酶联合消化法分离培养新生大鼠心肌telocytes,活细胞工作站动态捕捉和苏木素染色检测telocytes的分裂方式。结果酶联合消化法培养新生大鼠心肌telocytes纯度高、活力好,增殖迅速。无丝分裂时,胞核变大,以出芽方式生长,形成哑铃状细胞核,核芽逐渐拉伸并与母细胞分离。有丝分裂前中期,染色质缩短变粗为染色体,整齐地排列在赤道板处;分裂末期,染色体到达两极,子核已初步形成;胞质分裂期,胞质逐步拉开,形成两个子细胞。在有丝分裂不同时期,心肌telocytes均保持其原有的形态特征。结论体外培养心肌telocytes增殖时有丝分裂和无丝分裂两种方式并存。

Objective To explore the division mode of rat cardiac telocytes in vitro and to provide the morphological evidence for further research of telocytes. Methods Cardiac telocytes were isolated from neonatal rats and cultured with a combined type I collagenase （W/V, 0. 1% ） -trypsin （W/V, 0. 125% ） digestion method. The dynamic process of telocytes division was captured under a Live Cell Imaging System or with hematoxylin staining. Results Telocytes cultured with combined enzymes digestion were of high purity, survival for long time and rapid proliferation. Amitosis was observed in telocytes which has a large and budded nuclei. The dumbbell nucleus was then elongated and divided between the mother and daughter cells within the bud neck. Mitosis was characterized by the congregated chromosomes which were gradually shortened and coarsened, and nuclear chromatiu arranged tidily in the equatorial plane during prometaphase. Chrosomes were migrated to opposite poles and eventually the formation of the two daughter nuclei was completed during telophase. During cytokinesis, cytoplasm was pulled apart gradually and two new individual daughter cells came into being. Telocytes maintained their typical morphological features in different phases of mitosis. Conclusion There are two different division types in cardiac telocytes in vitro： namely amitosis and mitosis.