综合医院肺炎病人血清中嗜肺军团菌抗体调查

Legionella Pneumophila Antibody Investigation in Patients Serum with Pneumonia

目的调查我国三地区综合医院肺炎病人血清中嗜肺军团菌抗体水平。方法于2012年6月—2013年6月以北京、江苏和深圳3个地区的7家综合性医院的70名住院肺炎病人为研究对象,调查问卷收集基本信息,间接酶联免疫吸附法检测血清中嗜肺军团菌1—6血清型Ig G/Ig A/Ig M联合抗体。结果入院当天和入院两周后肺炎病人血清中嗜肺军团菌抗体阳性率分别为20%(7/35)和20%(7/35),其中5例肺炎病人双份血清均为阳性。结论我国综合医院肺炎病人存在嗜肺军团菌感染,采集入院当天和入院两周后血清同时进行嗜肺军团菌抗体检测可有效降低假阴性率。

Objective To investigate the infection of legionella pneumophila in patients with pneumonia in three districts of China. Methods Seventy hospitalized patients with pneumonia from seven general hospitals in Beijing,Jiangsu and Shenzhen were surveyed in Jun 2012 to Jun 2013. The general information of patients were inquired by questionnaire and the enzyme-linked immunosorbent assay was used for the combined detection of serum Ig G / Ig A / Ig M antibody for legionella pneumophila serotype 1—6. Blood samples of pneumonia patients were collected on the first day of admission and after two weeks of admission. Results The positive rate of serum legionella pneumophila antibody in pneumonia patients was 20%（ 7 /35） on the first day of admission,and was 20%（ 7 /35）after two weeks of admission. Positive serum legionella pneumophila antibody in both serum samples was detected only in 5 cases. Conclusions There was an infection of legionella pneumophila in pneumonia patients in China.False negative rate could be reduced effectively by the detection of serum legionella pneumophila antibodies with ELISA in pneumonia patients both on the day of admission and two weeks after admission.