利用高通量测序分析白花泡桐盐胁迫相关microRNAs

Analysis of salt stress-responsive microRNAs in Paulownia fortunei by high-throughput sequencing

为了鉴定和研究白花泡桐中与盐胁迫相关的microRNAs(miRNAs),构建了对照组PF2和处理组PF2-S4 2个小RNA文库。采用高通量测序技术总共鉴定出来了33个已知miRNA和80个新miRNA,在这些miRNAs中分别有27个(3个已知miRNA和24个新miRNA)差异显著表达和有45个(13个已知miRNA和32个新miRNA)差异极显著表达.最后采用qRT-PCR技术验证了5个miRNA。

To identify and investigate salt-related microRNAs （miRNAs） in Paulownia fortunei （P. fortunei）, we constructed and sequenced two small RNA libraries （PF2 and PF2-S4）. A total of 33 conserved and 80 novel miRNAs were identified in the two libraries by high-throughput sequencing. Among these miRNAs, 27 miRNAs （3 conserved and 24 novel） were differentially expressed and 45 miRNAs （13 conserved and 32 novel） were significantly differentially expressed in response to salt stress in P. fortunei, respectively. Finally, 5 of the miRNAs were confirmed by qRT-PCR.