E-cadherin、Ezrin在宫颈癌组织中的表达及临床意义

Expression and Clinical Significance of E-cadherin,Ezrin Protein in Cervical Cancer

目的 检测E-cadherin、Ezrin在正常宫颈组织、宫颈上皮内瘤变（CIN）及宫颈癌组织中的表达,探讨其在宫颈癌发生发展中的作用。方法 采用免疫组化SP法检测46例宫颈浸润癌、19例CINⅡ~Ⅲ、21例CINⅠ及20例正常宫颈组织中E-cadherin、Ezrin的表达,分析其临床意义。结果 在正常宫颈、CINⅠ、CINⅡ~Ⅲ及宫颈癌中,E-cadherin在胞膜的阳性表达率分别为95.0%、85.7%、68.4%、43.5%,呈逐步减弱或缺失表达,组间差异有统计学意义（P〈0.001）,而在胞质却逐渐增多。相反,Ezrin胞质阳性表达率分别为10.0%、28.6%、47.4%、73.9%,呈逐渐增高趋势,组间差异有统计学意义（P〈0.001）。Ezrin与E-cadherin在宫颈癌组织中的表达呈负相关（r=-0.458,P〈0.001）。Ezrin与E-cadherin在宫颈癌组织的阳性表达均与宫颈癌组织学分级、淋巴结转移相关,而与年龄、临床分期无关。结论 在CIN及宫颈癌组织E-cadherin在胞膜的表达呈逐步减弱或缺失,而Ezrin在胞质的表达却呈逐渐升高趋势,二者呈负相关。E-cadherin与Ezrin可能共同参与了宫颈癌的发生发展。

Objective To test the expression of E-cadherin and Ezrin in normal cervical epithelium, CIN and cervical cancer, and to investigate the role of E-cadherin and Ezrin in the progress of cervical cancer. Methods Immunohistochemical SP method was used to detect the expression of E-cadherin and Ezrin in 46 cases of cervical cancer, 19 cases of CIN II -III ,21 cases of CIN I , and 20 cases of normal cervical epithelium tissues, and their clinical significance analyzed. Results In the normal cervical epithelium,CIN I , CINIII III and cervical cancer, the cell membrane positive expression rate of E-cadherin were 95.0% ,85.7% ,68.4% , 43.5% , and the expression was gradually decreased or missing. There was significant difference among them （P 〈0. 001）, while the expression in cytoplasm was gradually increased. Oppositely, the cytoplasm positive expression rates of Ezrin were 10.0% ,28.6% ,47.4% ,73.9% , and the expression was gradually increased. There also was significant difference among them（P 〈 0. 001）. In cervical cancer,the expressions of Ezrin and E-cadherin were negative correlation （ r = 0. 458, P 〈 0. 001 ）. The expression of Ezrin and E-cadherin was significantly associated with tumor differention and lymphnode metastasis. Nevertheless,there was no significant association with clinical stages and age. Conclusion In CIN and cervical cancer, the expression of E- cadherin in cell membrane is gradually decreased or missing. However, Ezrin in cytoplasm is gradually increased,therefore E-cadherin and Ezrin may both participate in cervical cancer.