手足口病患者柯萨奇病毒核酸检测结果分析

Analysis on results of Coxsackie virus nucleic acid detection in HFMD patients

目的探讨手足口病患者柯萨奇病毒核酸检测的临床应用价值。方法利用实时荧光聚合酶链反应技术,以EV71/CA16基因组编码区的高度保守区为靶区域,设定特异性引物及荧光探针,通过一步法RT-PCR扩增对EV71RNA/CA16RNA进行检测。结果在428份患者标本中,EV71-RNA阳性136份,CA16-RNA阳性6份,其中有1例患者既是EV71-RNA咽拭子和肛拭子均阳性又是CA16-RNA咽拭子阳性。而有40例(80份标本)患者EV71-RNA咽拭子和肛拭子均阳性,46份EV71-RNA单独肛拭子阳性,10份EV71-RNA单独咽拭子阳性。在6份CA16-RNA阳性患者标本中,4份为肛拭子阳性,2份为咽拭子阳性。结论手足口病患者柯萨奇核酸检测对重症患者判断预后有重要的指导意义,对指导临床合理制订治疗方案有重要的参考价值,值得推广应用。

Objective To investigate the clinical application value of Coxsackie virus nucleic acid detection in the patients with hand‐foot‐mouth disease（HFMD） .Methods The real‐time fluorescent PCR technology was adoptd ,with a highly conserved region of EV71/CA16 genomic coding regionas the target area ,the specific primers and fluorescent probes were set ,EV71 RNA /CA16 RNA was detected by the one‐step method of RT‐PCR amplification . Results In 428 patients samples ,136 cases were EV71‐RNA positive ,6 cases were CA16‐RNA positive ,including 1 case of EV71‐RNA positive in throat swabs and anal swabs and CA16‐RNA positive in throat swab .And there were 40 cases （80 specimens） of EV71‐RNA positive in swabs and anal swabs ,46 cases of single EV71‐RNA positive in anal swab ,10 cases of single EV71‐RNA positive in throat swabs .In 6 cases of CA16‐RNA positive specimens ,4 cases were positive in anal swab ,two cases were positive in throat swab .Conclusion The Coxsackie nucleic acid testing in HFMD patients has an important guiding significance for judging the severe patients and their prognosis and has an important reference value for guiding clinic to formulate the treatment plan .