响应面分析用于槟榔储藏条件考察的研究

Application of Response Surface Analysis in the Investigation of Storage Condition of Areca catechu

探寻 A．flavus 菌株在槟榔原药材中生长及产黄曲霉毒素（AFB1，AFB2，AFG1和 AFG2）的规律，分析槟榔药材的最佳储藏条件。采用响应面分析法（Response Surface Analysis，RSA）结合 Central composite 设计，通过测定染菌后的槟榔中水分含量及总黄曲霉毒素的变化，考察温度（Temp，20～40℃）和湿度（Hum，80％～95％）两个环境因子对药材上 A．flavus菌株产毒的影响，分析该菌株的最适产毒条件。结果发现，储藏后的槟榔水分含量发生了显著的增加；当湿度范围为90％～95％，温度高于25℃时，A．flavus 菌株在槟榔中生长较快并产生大量黄曲霉毒素。研究表明，当储存条件为湿度小于90％，温度低于25℃，槟榔药材在储藏过程中不易霉变产毒，为槟榔储藏规范的制定及降低真菌毒素污染的概率提供了较好的依据。

The paper was to explore the law of growth of A.flavus and production of aflatoxins（AFB1 ,AFB2,AFG1 and AFG2） in raw Areca catechu and to study the optimal storage conditions of Areca catechu.Two environmental factors including temperature （temp,20 ~40 ℃）and humidity（Hum,80% ~95%）were investigated on the impact of the production of aflatoxins by A.flavus in medicinal herbs.The change of moisture content and the content of total aflatoxins after storage was tested through response sur-face analysis（RSA）combined with Central composite design,in order to analyze the environmental condition of the least aflatoxins production.The results showed that the moisture content of Areca catechu was significantly enhanced after storage.When the hu-midity was in range of 90% ~95% and temperature was higher than 25 ℃,A.flavus grew quickly and produced large amounts of aflatoxins on Areca catechu.The study showed that Areca catechu was not easy to mildew and produce toxins in environment with humidity less than 90%,temperature below 25 ℃.The result provided reference for establishing the optimal storage condition and for minimizing the possibility of aflatoxins contamination.