TDZ在4种观赏花卉芽增殖中的应用

TDZ Application on Bud Proliferation of Four Ornamental Flowers

为了探讨噻重氮苯基脲(TDZ)在4种观赏花卉(石斛兰,卡特兰、绿巨人、火鹤)芽增殖中的作用,提高芽增殖效率。试验在传统培养基基础上添加TDZ,分析不同浓度TDZ对4种观赏花卉芽增殖的影响。结果表明:石斛兰、卡特兰、绿巨人和火鹤芽增殖效果最好的培养基分别为:MS+6-BA 1.0 mg/L+NAA 0.1 mg/L+TDZ 0.04 mg/L、1/2MS+6-BA 0.2 mg/L+NAA 0.1 mg/L+TDZ 0.01 mg/L、MS+6-BA 3.0 mg/L+2,4-D 0.5 mg/L+TDZ 0.02 mg/L和MS+6-BA 1.5 mg/L+TDZ 0.04 mg/L。说明在4种观赏花卉芽增殖中添加TDZ能够提高分化效率。

In order to probe into the effect of TDZ application on bud proliferation of Dendrobium, Cattleya hybrida, Spathiphyllum pallas and Anthurium andraeanum and improve the proliferation efficiency, on the basis of the traditional medium, this paper analysed the different concentrations of TDZ＇s effect on bud proliferation of the four ornamenlal flower. The results showed that the best culture medium for bud proliferation of Dendrobium, Cattleya hybrida, Spathiphyllum pallas and Anthurium andraeanum was MS＋ 6-BA 1.0 mg/L＋ NAA 0.1 mg/L＋ TDZ 0.04 mg/ L, 1/2MS＋ 6-BA 0.2 mg/L＋ NAA 0.ling/L＋ TDZ 0.01mg/L, MS＋ 6-BA 3.0rag/L＋ 2,4-D 0.5rag/L＋ TDZ 0.02mg/L and MS＋ 6-BA 1.5mg/L＋ TDZ 0.04mg/L. This indicated that TDZ could improve the differentiation efficiency of four ornamental flowers.